Zanjan Metabolic Diseases Research Center, Zanjan University of Medical Sciences, Zanjan, Iran; Department of Nutrition, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Zanjan Metabolic Diseases Research Center, Zanjan University of Medical Sciences, Zanjan, Iran; Department of Obstetrics and Gynecology, IVF Clinic, Mousavi Hospital, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Int J Biol Macromol. 2021 Apr 30;177:157-165. doi: 10.1016/j.ijbiomac.2021.02.121. Epub 2021 Feb 18.
Although therapeutic effect of quercetin (Quer) was reported on non-alcoholic fatty liver disease (NAFLD), destructive effects have been shown on male fertility due to its pro-oxidative properties. On the other hand, NAFLD impairs germ cells to produce sperm and leads to male infertility. Herein, a biocompatible and green bigel was designed for Quer delivery to prevent infertility induced by NAFLD as the increasing complications. Bigels were prepared using cottonseed oil/cannabis oil/alginate/ferula gum and optimized by the mixture design method. NAFLD was induced by 58% of dietary calorie as lard and 42 g/l fructose for 16 weeks in Sprague-Dawley rats. So on animals received 2 mg/kg Quer loaded on bigels, free bigels, or free Quer for 45 days as daily gavage. Semen was analyzed, followed by the assessment of DNA integrity. Count, motility, and normal morphology reached the healthy control group at the bigel-Quer-treated one. Moreover, all of these parameters were significantly higher in the bigel-Quer group than the Quer and bigel, alone. The percent of sperms with head and tail abnormality decreased considerably in the bigel-Quer group compared with the Quer, free bigel, and NAFLD groups. Serum testosterone levels significantly increased and reached the healthy control group in the bigel-Quer group. DNA fragmentation of sperm significantly decreased in the bigel-Quer group (p < 0.05). The bigel showed synergistic effects with Quer for treating infertility in rats with NAFLD.
尽管槲皮素（Quer）在非酒精性脂肪性肝病（NAFLD）方面具有治疗效果，但由于其促氧化特性，已显示对男性生育力具有破坏性影响。另一方面，NAFLD 会损害生殖细胞产生精子，导致男性不育。本文中，设计了一种生物相容性和绿色的大凝胶用于 Quer 递送，以防止因 NAFLD 增加的并发症而导致的不育。大凝胶是使用棉籽油/大麻油/藻酸盐/阿魏胶制备的，并通过混合设计方法进行了优化。通过 58%的膳食卡路里作为猪油和 42g/L 果糖在 Sprague-Dawley 大鼠中诱导 16 周的 NAFLD。因此，在接受 2mg/kg Quer 负载于大凝胶、游离大凝胶或游离 Quer 每日灌胃 45 天的动物中。分析了精子，随后评估了 DNA 完整性。计数、活力和正常形态在大凝胶 Quer 治疗组中达到了健康对照组的水平。此外，在大凝胶 Quer 组中，所有这些参数均明显高于 Quer 和大凝胶单独组。与 Quer、游离大凝胶和 NAFLD 组相比，大凝胶 Quer 组的头部和尾部异常精子的比例显著降低。大凝胶 Quer 组的血清睾丸激素水平显著升高并达到健康对照组水平。精子 DNA 碎片化在大凝胶 Quer 组中显著降低（p<0.05）。大凝胶与 Quer 联合用于治疗 NAFLD 大鼠的不育症具有协同作用。
