Rehaman Sumayya, El-Sheikh Mohamed A, Alfarhan Ahamed H, Ushani U
Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore 641 021, Tamil Nadu, India.
Department of Botany & Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2021 Feb;28(2):1203-1212. doi: 10.1016/j.sjbs.2020.11.057. Epub 2020 Nov 24.
Microbial degradation as a treatment, with the combination of mixed inoculants of the Biofertilizer of sp.spi sp. and sp. was employed for the remediation of Silk dyeing effluent. Remediating studies was undertaken to assess the feasibility of the mixed biofertilizer inoculant source for degradation of the Azodye effluent from the Silk dyeing Industry. The Green leafy vegetable (GLV), used as investigational prototypical plant species is selected for examining the phytochemicals, functional groups and its compounds grown in the effluent and biotreated environment and compared. The laboratory scale investigation showed that leaves, stem and root of the was qualitatively analysed for 20 phytochemicals which was grown in the different treatments of raw effluent and the biotreated effluent and the results showed the phytochemicals on the effluent's influence reduced from strong positive to trace amounts while recovered on the biotreated environment. The FTIR analysis of the GLV grown in effluent and biotreated environments on comparison resulted in the functional group Alkene rescued in the biotreated effluent environment compared to the effluent contaminated area. The HPLC analysis of methanolic extracts of grown in fresh water has 6 peaks of retention time of 2.6, 3, 3.9, 4, 4.2, and 4.6 RT whereas GLV effluent had only one peak of retention time of 4.1 RT. In the GLV from biotreated environment have 4 peaks were found with the maximum percentage area of 95.2% which proves that the compounds are rescued in the biotreated environment and few active compounds were confirmed in GCMS analysis. The Soil analysis results also indicate that the biotreatment of mixed inoculant of biofertilizers in the biotreated soil had influence resulting in improved levels of Ca, N, P and K with 114, 213, 10.5, 268 kg/ha respectively in the mixed inoculant biotreated soil. Similarly the micronutrients suchas Fe, Mn, Cu and Zn ranges to 4.1, 20.22, 2.13, 1.13 ppm respectively in the mixed inoculant biotreated soil within the optimal range. The study revealed that mixed biofertilizer inoculant has the recovery effect on the Silk dyeing (Azodyes) effluents effective reducing the pollutant capacity thereby meeting the discharged standards.
采用微生物降解作为处理方法,将sp.spi sp.和sp.生物肥料的混合接种剂组合用于丝绸印染废水的修复。进行修复研究以评估混合生物肥料接种剂源对丝绸印染工业偶氮染料废水降解的可行性。选择用作研究原型植物物种的绿叶蔬菜(GLV),用于检测在废水和生物处理环境中生长的植物化学物质、官能团及其化合物,并进行比较。实验室规模的调查表明,对生长在原废水和生物处理后废水不同处理条件下的GLV的叶、茎和根进行了20种植物化学物质的定性分析,结果表明,废水中植物化学物质的影响从强阳性降低到痕量,而在生物处理环境中得以恢复。对生长在废水和生物处理环境中的GLV进行FTIR分析,结果表明,与受废水污染区域相比,生物处理后废水环境中挽救了烯烃官能团。对生长在淡水中的GLV甲醇提取物进行HPLC分析,有6个保留时间峰,分别为2.6、3、3.9、4、4.2和4.6 RT,而GLV废水只有一个保留时间峰,为4.1 RT。在生物处理环境中的GLV中发现有4个峰,最大百分比面积为95.2%,这证明化合物在生物处理环境中得以挽救,并且在GCMS分析中确认了少量活性化合物。土壤分析结果还表明,生物肥料混合接种剂在生物处理土壤中的生物处理产生了影响,导致混合接种剂生物处理土壤中Ca、N、P和K的含量分别提高到114、213、10.5、268 kg/ha。同样,混合接种剂生物处理土壤中的微量营养素如Fe、Mn、Cu和Zn分别为4.1、20.22、2.13、1.13 ppm,均在最佳范围内。研究表明,混合生物肥料接种剂对丝绸印染(偶氮染料)废水具有恢复作用,有效降低了污染物容量,从而达到排放标准。