高效液相色谱法（HPLC）检测遭受非生物胁迫的植物中的游离多胺

Detection of Free Polyamines in Plants Subjected to Abiotic Stresses by High-Performance Liquid Chromatography (HPLC).

作者信息

Gong Xiaoqing, Liu Ji-Hong

机构信息

College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070, Wuhan, China.

出版信息

Methods Mol Biol. 2017;1631:305-311. doi: 10.1007/978-1-4939-7136-7_19.

DOI:10.1007/978-1-4939-7136-7_19

PMID:28735406

Abstract

High-performance liquid chromatography (HPLC) is a sensitive, rapid, and accurate technique to detect and characterize various metabolites from plants. The metabolites are extracted with different solvents and eluted with appropriate mobile phases in a designed HPLC program. Polyamines are known to accumulate under abiotic stress conditions in various plant species and thought to provide protection against oxidative stress by scavenging reactive oxygen species. Here, we describe a common method to detect the free polyamines in plant tissues both qualitatively and quantitatively.

摘要

高效液相色谱法（HPLC）是一种用于检测和鉴定植物中各种代谢物的灵敏、快速且准确的技术。代谢物用不同溶剂提取，并在设计好的HPLC程序中用合适的流动相进行洗脱。已知多胺在各种植物物种的非生物胁迫条件下会积累，并被认为通过清除活性氧来提供对氧化胁迫的保护。在此，我们描述一种定性和定量检测植物组织中游离多胺的通用方法。