使用猪血清衍生的抗SARS-CoV-2抗体对SARS-CoV-2进行竞争性免疫测定。
Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies.
作者信息
Bong Ji-Hong, Kim Tae-Hun, Jung Jaeyong, Lee Soo Jeong, Sung Jeong Soo, Lee Chang Kyu, Kang Min-Jung, Kim Hyun Ok, Pyun Jae-Chul
机构信息
Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722 Republic of Korea.
Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), 5 Hwarang-ro 14-gil, Seongbuk-gu, Seoul, 02792 Republic of Korea.
出版信息
Biochip J. 2021;15(1):100-108. doi: 10.1007/s13206-021-00011-6. Epub 2021 Feb 17.
Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.
使用固定有人类严重急性呼吸综合征冠状病毒2（SARS-CoV-2）核蛋白（NP）的磁珠从猪血清中分离出抗SARS-CoV-2核蛋白抗体。使用固定有SARS-CoV-2 NP的磁珠通过流式细胞术检测分离出的抗体与SARS-CoV-2 NP的结合特性。开发了一种竞争性免疫测定法，用于使用固定有抗SARS-CoV-2 NP抗体的磁珠检测培养液中的SARS-CoV-2 NP以及SARS-CoV-2。在针对培养液中SARS-CoV、中东呼吸综合征冠状病毒（MERS-CoV）和冠状病毒株229E的竞争性免疫测定过程中进行了选择性测试。
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