Institute for Medical Microbiology and Virology, Leipzig University Hospital and Medical Faculty, University of Leipzig, Johannisallee 30, 04103, Leipzig, Germany.
Division of Infectious Diseases and Tropical Medicine, Department of Medicine II, Leipzig University Hospital, Leipzig, Germany.
Eur J Clin Microbiol Infect Dis. 2021 Dec;40(12):2645-2649. doi: 10.1007/s10096-021-04284-5. Epub 2021 Jun 4.
SARS CoV-2 antibody assays measure antibodies against the viral nucleoprotein (NP) or spike protein. The study examined if testing of antibodies against both antigens increases the diagnostic sensitivity. Sera (N=98) from infected individuals were tested with ELISAs based on the NP, receptor-binding domain (RBD), or both proteins. The AUROCs were 0.958 (NP), 0.991 (RBD), and 0.992 (NP/RBD). The RBD- and NP/RBD-based ELISAs showed better performance than the NP-based assay. Simultaneous testing for antibodies against NP and RBD increased the number of true and false positives. If maximum diagnostic sensitivity is required, the NP/RBD-based ELISA is preferable. Otherwise, the RBD-based ELISA is sufficient.
SARS-CoV-2 抗体检测试剂盒可检测针对病毒核衣壳蛋白（NP）或刺突蛋白的抗体。本研究旨在探讨针对这两种抗原的抗体检测是否能提高诊断灵敏度。采用基于 NP、受体结合域（RBD）或两者的 ELISA 法检测感染个体的血清（N=98）。NP、RBD 和 NP/RBD 的 AUROC 分别为 0.958、0.991 和 0.992。基于 RBD 和 NP/RBD 的 ELISA 比基于 NP 的 ELISA 具有更好的性能。同时检测针对 NP 和 RBD 的抗体增加了真阳性和假阳性的数量。如果需要最大的诊断灵敏度，基于 NP/RBD 的 ELISA 更可取。否则，基于 RBD 的 ELISA 就足够了。
