Niu Yingfeng, Gao Chengwen, Liu Jin
Yunnan Institute of Tropical Crops, Xishuangbanna, China.
The Affiliated Hospital of Qingdao University, Qingdao, China.
PeerJ. 2021 Feb 10;9:e10774. doi: 10.7717/peerj.10774. eCollection 2021.
Mango is an important commercial fruit crop belonging to the genus Mangifera. In this study, we reported and compared four newly sequenced plastid genomes of the genus Mangifera, which showed high similarities in overall size (157,780-157,853 bp), genome structure, gene order, and gene content. Three mutation hotspots (, and ) were identified as candidate DNA barcodes for Mangifera. These three DNA barcode candidate sequences have high species identification ability. We also identified 12 large fragments that were transferred from the plastid genome to the mitochondrial genome, and found that the similarity was more than 99%. The total size of the transferred fragment was 35,652 bp, accounting for 22.6% of the plastid genome. Fifteen intact chloroplast genes, four tRNAs and numerous partial genes and intergenic spacer regions were identified. There are many of these genes transferred from mitochondria to the chloroplast in other species genomes. Phylogenetic analysis based on whole plastid genome data provided a high support value, and the interspecies relationships within Mangifera were resolved well.
芒果是芒果属的一种重要商业水果作物。在本研究中，我们报道并比较了芒果属四个新测序的质体基因组，它们在总体大小（157,780 - 157,853 bp）、基因组结构、基因顺序和基因含量方面表现出高度相似性。确定了三个突变热点（、和）作为芒果属的候选DNA条形码。这三个DNA条形码候选序列具有较高的物种鉴定能力。我们还鉴定出12个从质体基因组转移到线粒体基因组的大片段，发现其相似度超过99%。转移片段的总大小为35,652 bp，占质体基因组的22.6%。鉴定出15个完整的叶绿体基因、4个tRNA以及众多部分基因和基因间隔区。在其他物种基因组中有许多此类基因从线粒体转移到叶绿体。基于全质体基因组数据的系统发育分析提供了较高的支持值，并且很好地解析了芒果属内的种间关系。
