Liu Xinyu, Zhou Lirong, Du Chengxun, Wang Songbiao, Chen Hongjin, Xu Wentian, Yang Zhuanying, Liang Qingzhi
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
Key Laboratory of Disaster Weather Defense and Climate Resource Utilization of Panzhihua City, Renhe Meteorological Bureau of Panzhihua, Panzhihua 617000, China.
Metabolites. 2024 Oct 11;14(10):543. doi: 10.3390/metabo14100543.
The dramatic temperature fluctuations spurred by global warming and the accompanying extreme weather events inhibit mango growth and threaten mango productivity. Particularly, mango flowering is highly sensitive to temperature changes. The mango fruit setting rate was significantly positively correlated with pollen activity, and pollen activity was regulated by different metabolites.
In this study, the in vitro pollen of two mango varieties ('Renong No.1' and 'Jinhuang'), in which sensitivity to temperature differed significantly, were subjected to different temperature stresses (15 °C, 25 °C and 35 °C), and their metabolomics were analyzed.
The present results showed that 775 differential metabolites were screened by liquid chromatography-mass spectrometry and divided into 12 categories. The two varieties had significant differences in metabolite expression under different temperature stresses and the effect of low temperature on 'Renong No.1' mainly focused on amino acid metabolism, while the effect on 'Jinhuang' was mainly related to glycolysis. However, under the 35 °C temperature stress, 'Renong No.1' responded by redistributing riboflavin and betaine in vivo and the most obvious metabolic pathway of 'Jinhuang' enrichment was pyrimidine metabolism, which had undergone complex main body formation and extensive regulatory processes. The changes of metabolites of different varieties under low temperature and high temperature stress were different. Among them, flavonoids or flavonoid derivatives were included in class A (216 metabolites), C (163 metabolites) and D (233 metabolites) metabolites, indicating that flavonoid metabolites had an obvious regulatory effect on mango pollen metabolism under different temperature stress.
The present results provide valuable information for reproductive biology studies and breeding in mango, in particular, the selection and breeding of the most suitable varieties for different production areas.
全球变暖引发的剧烈温度波动以及随之而来的极端天气事件抑制了芒果生长，威胁着芒果产量。特别是，芒果开花对温度变化高度敏感。芒果坐果率与花粉活性显著正相关，且花粉活性受不同代谢物调控。
本研究对两个温度敏感性差异显著的芒果品种（“热农一号”和“金黄芒”）的离体花粉进行不同温度胁迫（15℃、25℃和35℃）处理，并分析其代谢组学。
液相色谱-质谱联用筛选出775种差异代谢物，分为12类。两个品种在不同温度胁迫下代谢物表达存在显著差异，低温对“热农一号”的影响主要集中在氨基酸代谢，而对“金黄芒”的影响主要与糖酵解有关。然而，在35℃温度胁迫下，“热农一号”通过在体内重新分配核黄素和甜菜碱做出响应，“金黄芒”富集最明显的代谢途径是嘧啶代谢，该代谢途径经历了复杂的主体形成和广泛的调控过程。不同品种在低温和高温胁迫下代谢物变化不同。其中，A类（216种代谢物）、C类（163种代谢物）和D类（233种代谢物）代谢物中均包含黄酮类或黄酮类衍生物，表明黄酮类代谢物在不同温度胁迫下对芒果花粉代谢具有明显调控作用。
本研究结果为芒果生殖生物学研究和育种，特别是为不同产区选择和培育最合适品种提供了有价值的信息。
