Center for Vaccines and Immunology, University of Georgia, Athens, GA, United States of America.
Atreca, Inc., South San Francisco, CA, United States of America.
PLoS One. 2021 Feb 22;16(2):e0247253. doi: 10.1371/journal.pone.0247253. eCollection 2021.
Recent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B cell receptor (BCR) repertoires. In this study, PBMCs were collected from 17 human participants vaccinated with the split-inactivated influenza virus vaccine during the 2016-2017 influenza season. A combination of Immune Repertoire Capture (IRCTM) technology and IgG sequencing was performed on ~7,800 plasmablast (PB) cells and preferential IgG heavy-light chain pairings were investigated. In some participants, a single expanded clonotype accounted for ~22% of their PB BCR repertoire. Approximately 60% (10/17) of participants experienced convergent evolution, possessing public PBs that were elicited independently in multiple participants. Binding profiles of one private and three public PBs confirmed they were all subtype-specific, cross-reactive hemagglutinin (HA) head-directed antibodies. Collectively, this high-resolution antibody repertoire analysis demonstrated the impact evolution can have on BCRs in response to influenza virus vaccination, which can guide future universal influenza prophylactic approaches.
高通量单细胞测序的最新进展为研究 B 细胞受体 (BCR) 库开辟了新途径。在这项研究中，从 17 名在 2016-2017 流感季节接种流感病毒裂解疫苗的人类参与者中采集 PBMC。对约 7800 个浆母细胞 (PB) 细胞进行了免疫受体捕获 (IRCTM) 技术和 IgG 测序的组合，并研究了优先 IgG 重轻链配对。在一些参与者中，单一扩展克隆型占其 PB BCR 库的~22%。大约 60%（17 人中的 10 人）经历了趋同进化，具有在多个参与者中独立诱导的公共 PB。一个私有和三个公共 PB 的结合谱证实它们都是亚型特异性的、交叉反应的血凝素 (HA) 头部定向抗体。总的来说，这项高分辨率抗体库分析表明，进化对流感病毒疫苗接种后 BCR 产生的影响，可以指导未来通用流感预防方法。
