Matsumura H, Daikoku S
Cell Tissue Res. 1977 Sep 5;182(4):541-8. doi: 10.1007/BF00219836.
Localization and number of pituitary LH-cells were studied in neonatal male and female rats (from the birth to 12th day) applying anti-HCG serum in immunoenzymological procedures. The cells increased in number with developing age after birth. The cells in males and females were equal in number until 4 days of age, wheres thereafter the increase of the cell number in females exceeded that in males. After birth, the cells are mainly concentrated ventrally, being ventro-lateral in the anterior region but converging into the medial-ventral area in the posterior part of the gland. Some dispersion in a dorsal direction is also noted in the latter region. At birth the cells begin to appear in the dorsal area in the anterior portion, as well as in the posterior portion, particularly in the area close to the intermediate lobe and in the zone adjacent ot the residual lumen. This was particularly evident in females after 4 days of age. Thus it is concluded that in rats the sexual differences in the pituitary become apparent after the 4th day of postnatal life.
应用抗人绒毛膜促性腺激素（HCG）血清，通过免疫酶学方法研究了新生雄性和雌性大鼠（出生至第12天）垂体促黄体生成素（LH）细胞的定位和数量。出生后，这些细胞的数量随着年龄增长而增加。出生后4天之前，雄性和雌性的细胞数量相等，但此后雌性细胞数量的增加超过雄性。出生后，这些细胞主要集中在腹侧，在前部区域为腹外侧，但在腺体后部汇聚到内侧腹侧区域。在该区域的后部也观察到一些向背侧的分散。出生时，这些细胞开始出现在前部的背侧区域以及后部，特别是靠近中间叶的区域和与残留管腔相邻的区域。这在出生后4天的雌性大鼠中尤为明显。因此得出结论，大鼠垂体的性别差异在出生后第4天变得明显。
