骨形态发生蛋白 7 在自体血、冻干骨和去端胶原辅助牙槽骨增量中的表达。
Bone Morphogenetic Protein 7 Expression in Alveolar Bone Addition With Autologous Blood, Lyophilized Bone and Atelocollagen.
机构信息
Discipline of Medical Hygiene, "Iuliu Hațieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Surgical Pathology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania.
出版信息
In Vivo. 2021 Mar-Apr;35(2):871-881. doi: 10.21873/invivo.12327.
BACKGROUND/AIM: The biomaterials used in guided bone regeneration have undergone significant diversification in recent years. This study aimed to evaluate alveolar bone addition and bone morphogenetic protein 7 (BMP7) expression using an improved autologous and xenogeneic biomaterial.
MATERIALS AND METHODS
Chronic marginal periodontitis was induced in sheep; the intervention group received bone addition as periodontal therapy, using a composite system with lyophilized bovine bone enriched with atelocollagen type 1, platelet-rich plasma and advanced platelet-rich fibrin (A-PRF). Six weeks after the intervention, the dentoalveolar structures were evaluated using hematoxylin-eosin and immunohistochemical staining, to evaluate bone addition and BMP7 expression.
RESULTS
The untreated sheep showed inflammation, periodontal ligament destruction, remnants of calculus and bacterial plaque as well as foreign bodies in the desmodontal space, without sings of repair. In the treated sheep, fibroblasts/fibrosis, cartilage and/or new bone, cellular cementum and desmodontium, along with remnants of biomaterial with various degrees of cellularity were observed. In the untreated group, the presence of BMP7 was found in osteoblasts and osteocytes while in the treated group, it was mainly found in the biomaterial remnants, while immunohistochemical staining was less intense in the newly formed osteo-periodontal tissues. Quantitative analysis using the Mann-Whitney U-test showed highly statistically significant differences between the two groups, demonstrating the efficiency of this composite system.
CONCLUSION
The current composite system meets all the necessary conditions for promising guided alveolar bone regeneration.
背景/目的：近年来，用于引导骨再生的生物材料发生了显著的多样化。本研究旨在评估一种改良的自体和异种生物材料的牙槽骨增量和骨形态发生蛋白 7（BMP7）表达。
材料和方法
在绵羊中诱导慢性边缘性牙周炎；干预组接受骨增量作为牙周治疗，使用一种复合系统，该系统由富含 1 型胶原的冻干牛骨、富含血小板的血浆和先进的富含血小板纤维蛋白（A-PRF）组成。干预 6 周后，使用苏木精-伊红和免疫组织化学染色评估牙牙槽结构，以评估骨增量和 BMP7 表达。
结果
未经治疗的绵羊表现出炎症、牙周韧带破坏、牙石残片和细菌斑块以及牙骨质空间中的异物，没有修复迹象。在治疗组的绵羊中，观察到成纤维细胞/纤维化、软骨和/或新骨、细胞性牙骨质和牙骨质、以及具有不同程度细胞性的生物材料残余物。在未治疗组中，BMP7 存在于成骨细胞和成骨细胞中，而在治疗组中，BMP7 主要存在于生物材料残余物中，而新形成的牙周组织中的免疫组织化学染色较弱。使用曼-惠特尼 U 检验的定量分析显示两组之间存在高度统计学差异，表明这种复合系统的效率。
结论
目前的复合系统满足了有前途的引导牙槽骨再生的所有必要条件。
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