Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi 110016, India.
J Mater Chem B. 2021 Mar 17;9(10):2436-2446. doi: 10.1039/d0tb02842h.
Rapid and selective detection of microorganisms in complex biological systems draws huge attention to address the rising issue of antimicrobial resistance. Diagnostics based on the identification of whole microorganisms are laborious, time-consuming and costly, thus alternative strategies for early clinical diagnosis include biomarker based microbial detection. This paper describes a low-cost, easy-to-use method for the detection of Pseudomonas aeruginosa infections by specifically identifying a biomarker pyocyanin, using surface-molecularly imprinted nanoparticles or "plastibodies". The selective nanopockets are created by templating pyocyanin onto 20 nm allyl-functionalized magnetic nanoparticles coated with a thin layer of the acrylamide-based polymer. This functional material with an impressive imprinting factor (IF) of 5 and a binding capacity of ∼2.5 mg g-1 of polymers can be directly applied for the detection of bacteria in complex biological samples based on the presence of pyocyanin. These MIPs are highly selective and sensitive to pyocyanin and can consistently bind with pyocyanin in repeated use. Finally, the facile and efficient capture of pyocyanin has versatile applications ranging from biomarker based culture free detection of P. aeruginosa to monitoring of the therapeutic regime, in addition to developing a new class of antibiotics.
快速且选择性地检测复杂生物系统中的微生物，引起了人们对日益严重的抗微生物药物耐药性问题的关注。基于对整个微生物进行鉴定的诊断方法既费力、耗时又昂贵，因此，用于早期临床诊断的替代策略包括基于生物标志物的微生物检测。本文描述了一种低成本、易于使用的方法，用于通过特异性识别生物标志物绿脓菌素来检测铜绿假单胞菌感染，使用表面分子印迹纳米颗粒或“plastibodies”。通过将绿脓菌素模板化到 20nm 烯丙基功能化的磁性纳米颗粒上，并在其表面涂覆一层薄的丙烯酰胺基聚合物，形成了选择性纳米口袋。这种功能材料具有令人印象深刻的印迹因子（IF）为 5 和结合能力约为 2.5mg g-1 聚合物，可以直接应用于基于绿脓菌素存在的复杂生物样品中细菌的检测。这些 MIP 对绿脓菌素具有高度选择性和敏感性，并且可以在重复使用中一致地结合绿脓菌素。最后，绿脓菌素的简便高效捕获具有多种应用，从基于生物标志物的无需培养的铜绿假单胞菌检测到治疗方案的监测，此外还开发了一类新的抗生素。
