Ejaz Samina, Nasim Faiz-Ul-Hassan, Abdullah Iqra, Rashid Samia, Ashraf Muhammad
Department of Biochemistry, Institute of Biochemistry, Biotechnology and Bioinformatics (IBBB), The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
Institute of Biochemistry, Biotechnology and Bioinformatics (IBBB), The Islamia University of Bahawalpur, 63100, Pakistan.
Saudi J Biol Sci. 2023 Sep;30(9):103750. doi: 10.1016/j.sjbs.2023.103750. Epub 2023 Jul 28.
This study aimed to investigate the antibacterial and cytotoxic activity of 03 medicinal plants, , and from Cholistan desert, Pakistan. The active constituents of plants species were extracted in 05 different solvents and the extracts were tested against various bacterial strains and brine shrimps. Although all 's extracts except chloroform were active against the most active was the acetone extract (21 ± 0.00 mm at 200 μg/disc) and activity was better than Caricef (p-value 0.03). While its water extract was more potent (18 ± 1.45 mm at 200 μg/disc) than Augmentin and Caricef (p-value < 0.005). The methanol extract's activity (15 ± 0.39 mm in 200 μg/disc) was comparable to Fucidin against (p-value > 0.99) and activity of diethyl ether extract against (10 ± 1.16 mm in 200 μg/disc) was same as of Urixin (p-value 0.91)'s acetone extract against (10 ± 0.15 mm in 1 μg/disc) was more active than Augmentin Caricef and Cefotax (p-value < 0.02) and against (15 ± 1.15 mm in 200 μg/disc) activity was higher than Caricef (p-value 0.03). All 's extracts except water extract were found active against However, the diethyl ether extract was most effective (25 + 0.00 mm at 150 μg /disc) and activity was more than Augmentin, Oxy-tetracycline, Fucidin, Urixin, Ceftriaxone (p-value < 0.05). Although all extracts were exhibited cytotoxic activity, the 's acetone extract (100%), 's diethyl ether extract (90%) and 's methanol extract (100%) were most active at 1000 μg/ml concentration. This study validated the medicinal significance of the studied plants and thus opens the way for their therapeutic applications.
本研究旨在调查来自巴基斯坦乔利斯坦沙漠的3种药用植物（此处植物名称缺失）的抗菌和细胞毒性活性。将植物物种的活性成分用5种不同溶剂进行提取，并对提取物针对各种细菌菌株和卤虫进行测试。尽管除氯仿提取物外，所有（此处植物名称缺失）的提取物对（此处细菌名称缺失）均有活性，但活性最强的是丙酮提取物（在200μg/圆盘时为21±0.00mm），其活性优于卡立cef（p值为0.03）。而其水提取物更有效（在200μg/圆盘时为18±1.45mm），优于阿莫西林克拉维酸钾和卡立cef（p值<0.005）。甲醇提取物的活性（在200μg/圆盘时为15±0.39mm）与夫西地酸对（此处细菌名称缺失）的活性相当（p值>0.99），二乙醚提取物对（此处细菌名称缺失）的活性（在200μg/圆盘时为10±1.16mm）与Urixin相同（p值为0.91）。（此处植物名称缺失）的丙酮提取物（在1μg/圆盘时为10±0.15mm）对（此处细菌名称缺失）的活性比阿莫西林克拉维酸钾、卡立cef和头孢噻肟更具活性（p值<0.02），对（此处细菌名称缺失）的活性（在200μg/圆盘时为15±1.15mm）高于卡立cef（p值为0.03）。除水提取物外，所有（此处植物名称缺失）的提取物对（此处细菌名称缺失）均有活性。然而，二乙醚提取物最有效（在150μg/圆盘时为25 + 0.00mm），其活性高于阿莫西林克拉维酸钾、土霉素、夫西地酸、Urixin、头孢曲松（p值<0.05）。尽管所有提取物均表现出细胞毒性活性，但（此处植物名称缺失）的丙酮提取物（100%）、（此处植物名称缺失）的二乙醚提取物（90%）和（此处植物名称缺失）的甲醇提取物（100%）在1000μg/ml浓度下活性最强。本研究验证了所研究植物的药用意义，从而为其治疗应用开辟了道路。
