Hernández-Huerta Jared, Tamez-Guerra Patricia, Gomez-Flores Ricardo, Delgado-Gardea Ma Carmen E, García-Madrid Margarita S, Robles-Hernández Loreto, Infante-Ramirez Rocio
Facultad de Ciencias Agrotecnológicas, Universidad Autónoma de Chihuahua, Campus 1, Chihuahua, Chihuahua, México.
Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México.
PeerJ. 2021 Feb 15;9:e10913. doi: 10.7717/peerj.10913. eCollection 2021.
spp. causes bacterial spot disease, which reduces quality and yield of pepper crops in Mexico. Identification of phytopathogen species is necessary to implement more effective control strategies.
The aim of this study was to isolate and identify infecting species in South Central Chihuahua pepper-producing areas.
Diseased plants were collected from 30 cultivation lots and bacteria were isolated from damaged tissues. Potential causative agents were isolated, identified, and characterized by biochemical and molecular analysis. Pathogenicity tests from each isolate were then performed on 30-d-old pepper seedlings, exposing five leaves to 10 µL of 1 × 10 CFU/mL bacterial suspensions of each isolate, using sterile distilled water as a control. Disease severity was determined after 10 d by calculating leaf damage percentage. Furthermore, we evaluated the susceptibility of the highest bacterial spot severity-causing isolates (13 isolates) to copper sulphate (CuS), copper gluconate (CuG), copper oxychloride + oxytetracycline hydrochloride (Cu + Ox), gentamicin + oxytetracycline hydrochloride (Gen + Ox), and gentamicin sulphate (GenS). Copper-resistance genes () were detected by PCR analysis among isolates.
Thirty-seven foliage isolates were identified as (14%), which were associated with bacterial spot disease in jalapeño pepper. Tested isolates were resistant to Cu-based compounds, but susceptible to Cu + Ox. All isolates were susceptible to Gen + Ox and GenS. genes were detected in all but one strain.
(formally ) may be considered as an emerging pathogen of bacterial spot pepper in Mexico. Among disease management strategies, alternatives to copper should be taken into consideration.
[病原菌名称]引起细菌性斑点病，该病会降低墨西哥辣椒作物的品质和产量。识别植物病原体种类对于实施更有效的控制策略至关重要。
本研究的目的是在奇瓦瓦州中南部辣椒产区分离并鉴定感染[病原菌名称]的种类。
从30个种植地块采集患病植株，从受损组织中分离细菌。通过生化和分子分析对潜在病原体进行分离、鉴定和表征。然后对30日龄的辣椒幼苗进行每个分离株的致病性测试，将每株分离株的10 μL 1×10 CFU/mL细菌悬液接种到五片叶子上，以无菌蒸馏水作为对照。10天后通过计算叶片损伤百分比来确定病害严重程度。此外，我们评估了引起细菌性斑点病严重程度最高的分离株（13个分离株）对硫酸铜（CuS）、葡萄糖酸铜（CuG）、氢氧化铜+盐酸土霉素（Cu + Ox）、庆大霉素+盐酸土霉素（Gen + Ox）和硫酸庆大霉素（GenS）的敏感性。通过PCR分析在分离株中检测铜抗性基因（）。
37个叶片分离株被鉴定为[病原菌名称]（14%），它们与墨西哥胡椒的细菌性斑点病有关。测试的[病原菌名称]分离株对铜基化合物具有抗性，但对Cu + Ox敏感。所有分离株对Gen + Ox和GenS敏感。除一个菌株外，在所有菌株中均检测到[铜抗性基因名称]基因。
[病原菌名称]（正式名称为[原名]）可能被视为墨西哥辣椒细菌性斑点病的一种新兴病原体。在病害管理策略中，应考虑使用替代铜制剂的方法。
