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[植物名称]种子甲醇提取物对大鼠胚胎和胎儿的毒性

Toxicity of Methanolic Extracts of Seeds of , in Rat Embryos and Fetuses.

作者信息

Teshome Daniel, Tiruneh Chalachew, Berihun Gete

机构信息

Department of Anatomy, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.

Department of Environmental Health, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.

出版信息

Biomed Res Int. 2021 Feb 9;2021:5291083. doi: 10.1155/2021/5291083. eCollection 2021.

Abstract

is a medicinal plant that has been used in Ethiopian traditional medicine as a remedy for the treatment of hypertension, diabetes, and stomach pain. The study is aimed at assessing the toxicity of the methanol extracts of the seeds of on the developing embryo and fetuses of rats. The seeds of were extracted by maceration using 80% methanol. The extract (250-1000 mg/kg) was orally administered to pregnant Swiss albino rats from days 6 to12 of gestation. Embryos and fetuses were recovered by laparotomy on gestational day 12 and day 20, respectively, and were assessed for developmental anomalies. On day 20, significant prenatal growth retardation such as reduced litter weight and crown-rump length were observed in near term fetuses of 1000 mg/kg treated rats. Litter weight in 1000 mg/kg and pair-fed control groups was 2.41 g ± 0.108 and 3.08 g ± 0.093, respectively. Delay in the development of an otic, optic, and olfactory system, as well as a reduction in a number of branchial bars, occurred on day 12 embryos of 1000 mg/kg treated rats. The rate of fetal resorption in 1000 mg/kg and pair-fed control groups was 1.6 ± 0.55 and 0.42 ± 0.52, respectively. There was also a high incidence of fetal death in the 1000 mg/kg treated group but it was not statistically significant. The offspring's of -treated rats did not show gross external malformations at all doses. These findings suggest that the methanol seed extract of is not safe to rat embryos and fetuses. Its toxic effects were evidenced by a significant delay in embryonic and fetal development and an increase in fetal resorptions and fetal death.

摘要

是一种药用植物,在埃塞俄比亚传统医学中被用作治疗高血压、糖尿病和胃痛的药物。该研究旨在评估其种子甲醇提取物对大鼠发育中的胚胎和胎儿的毒性。其种子用80%甲醇浸渍提取。提取物(250 - 1000毫克/千克)在妊娠第6天至12天经口给予怀孕的瑞士白化大鼠。分别在妊娠第12天和第20天通过剖腹术取出胚胎和胎儿,并评估其发育异常情况。在第20天,在接受1000毫克/千克处理的大鼠的近足月胎儿中观察到明显的产前生长迟缓,如窝仔体重和顶臀长度减少。1000毫克/千克组和配对喂养对照组的窝仔体重分别为2.41克±0.108和3.08克±0.093。在接受1000毫克/千克处理的大鼠的第12天胚胎中,出现了耳、眼和嗅觉系统发育延迟以及鳃弓数量减少的情况。1000毫克/千克组和配对喂养对照组的胎儿吸收发生率分别为1.6±0.55和0.42±0.52。在1000毫克/千克处理组中胎儿死亡发生率也很高,但无统计学意义。在所有剂量下,该植物处理的大鼠后代均未表现出明显的外部畸形。这些发现表明,该植物种子甲醇提取物对大鼠胚胎和胎儿不安全。其毒性作用表现为胚胎和胎儿发育明显延迟以及胎儿吸收和胎儿死亡增加。

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