Department of pharmacology and clinical pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
Aklilu Lemma institute of pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
BMC Complement Altern Med. 2017 Sep 29;17(1):473. doi: 10.1186/s12906-017-1982-y.
Many people still experience pain and inflammation regardless of the available drugs for treatments. In addition, the available drugs have many side effects, which necessitated a quest for new drugs from several sources in which medicinal plants are the major one. This study evaluated the analgesic and anti- inflammatory activity of the solvent fractions of Moringa stenopetala in rodent models of pain and inflammation.
Successive soxhlet and maceration were used as methods of extractions using solvents of increasing polarity; chloroform, methanol and water. Swiss albino mice models were used in radiant tail flick latency, acetic acid induced writhing and carrageenan induced paw edema to assess the analgesic and anti-inflammatory activities. The test groups received different doses (100 mg/kg, 200 mg/kg and 400 mg/kg) of the three fractions (chloroform, methanol and aqueous). The positive control groups received morphine (20 mg/kg) or aspirin (100 mg/kg or 150 mg/kg) based on the respective models. The negative control groups received the 10 ml/kg of vehicles (distilled water or 2% Tween 80).
In all models, the chloroform fraction had protections only at a dose of 400 mg/kg. However, the methanol and aqueous fraction at all doses have shown significant central and peripheral analgesic activities with a comparable result to the standards. The aqueous and methanol fractions significantly reduced carrageenan induced inflammation in a dose dependent manner, in which the highest reduction of inflammation was observed in aqueous fraction at 400 mg/kg.
This study provided evidence on the traditionally claimed uses of the plant in pain and inflammatory diseases, and Moringa stenopetala could be potential source for development of new analgesic and anti-inflammatory drugs.
尽管有可用的治疗药物，但许多人仍会经历疼痛和炎症。此外，现有的药物有许多副作用，这就需要从多种来源寻找新药，其中药用植物是主要来源。本研究评估了溶剂提取物对 Moringa stenopetala 在疼痛和炎症的啮齿动物模型中的镇痛和抗炎活性。
采用索氏提取法和浸渍提取法作为提取方法，使用的溶剂极性逐渐增加，分别为氯仿、甲醇和水。采用瑞士白化鼠的辐射尾部闪烁潜伏期、醋酸诱导扭体和角叉菜胶诱导足肿胀模型来评估镇痛和抗炎活性。实验组分别给予三种馏分（氯仿、甲醇和水）的不同剂量（100mg/kg、200mg/kg 和 400mg/kg）。阳性对照组分别给予吗啡（20mg/kg）或阿司匹林（100mg/kg 或 150mg/kg），根据各自的模型而定。阴性对照组给予 10ml/kg 的载体（蒸馏水或 2%吐温 80）。
在所有模型中，氯仿馏分仅在 400mg/kg 剂量下具有保护作用。然而，甲醇和水馏分在所有剂量下均表现出显著的中枢和外周镇痛活性，与标准品相当。水馏分和甲醇馏分均能剂量依赖性地减少角叉菜胶诱导的炎症，其中在 400mg/kg 剂量下水馏分的抗炎作用最强。
本研究为该植物在疼痛和炎症性疾病中的传统应用提供了证据，Moringa stenopetala 可能是开发新型镇痛和抗炎药物的潜在来源。
