School of Chemistry, Sun Yat-sen University, Guangzhou, P. R. China.
J Sep Sci. 2021 Apr;44(8):1752-1768. doi: 10.1002/jssc.202001196. Epub 2021 Mar 13.
Surface-enhanced Raman spectroscopy is a significant analytical tool capable of fingerprint identification of molecule in a rapid and ultrasensitive manner. However, it is still hard to meet the requirements of practical sample analysis. The introduction of microfluidics can effectively enhance the performance of surface-enhanced Raman spectroscopy in complex sample analysis including reproducibility, selectivity, sensitivity, and speed. This review summarizes the recent progress of microfluidics in surface-enhanced Raman spectroscopic analysis through four combination approaches. First, microfluidic synthetic techniques offer uniform nano-/microparticle fabrication approaches for reproductive surface-enhanced Raman spectroscopic analysis. Second, the integration of microchip and surface-enhanced Raman spectroscopic substrate provides advanced devices for sensitive and efficient detection. Third, microfluidic sample preparations enable rapid separation and preconcentration of analyte prior to surface-enhanced Raman spectroscopic detection. Fourth, highly integrated microfluidic devices can be employed to realize multistep surface-enhanced Raman spectroscopic analysis containing material fabrication, sample preparation, and detection processes. Furthermore, the challenges and outlooks of the application of microfluidics in surface-enhanced Raman spectroscopic analysis are discussed.
表面增强拉曼光谱是一种重要的分析工具，能够快速、灵敏地对分子进行指纹识别。然而，它仍然难以满足实际样品分析的要求。微流控技术的引入可以有效地增强表面增强拉曼光谱在复杂样品分析中的性能，包括重现性、选择性、灵敏度和速度。本文通过四种组合方法综述了微流控技术在表面增强拉曼光谱分析中的最新进展。首先，微流控合成技术为重复性表面增强拉曼光谱分析提供了均匀的纳米/微颗粒制备方法。其次，微芯片和表面增强拉曼光谱基底的集成提供了用于灵敏和高效检测的先进器件。第三，微流体样品制备能够在表面增强拉曼光谱检测之前快速分离和浓缩分析物。第四，高度集成的微流控器件可用于实现包含材料制备、样品制备和检测过程的多步表面增强拉曼光谱分析。此外，还讨论了微流控在表面增强拉曼光谱分析中的应用所面临的挑战和展望。
