Institute of Analytical Chemistry, Leipzig University, Linnéstraße 3, 04103 Leipzig, Germany.
Department of Chemistry, SRM University AP, Amaravati, Andhra Pradesh 522502, India.
Lab Chip. 2022 Feb 15;22(4):665-682. doi: 10.1039/d1lc01097b.
With the continuous development in nanoscience and nanotechnology, analytical techniques like surface-enhanced Raman spectroscopy (SERS) render structural and chemical information of a variety of analyte molecules in ultra-low concentration. Although this technique is making significant progress in various fields, the reproducibility of SERS measurements and sensitivity towards small molecules are still daunting challenges. In this regard, microfluidic surface-enhanced Raman spectroscopy (MF-SERS) is well on its way to join the toolbox of analytical chemists. This review article explains how MF-SERS is becoming a powerful tool in analytical chemistry. We critically present the developments in SERS substrates for microfluidic devices and how these substrates in microfluidic channels can improve the SERS sensitivity, reproducibility, and detection limit. We then introduce the building materials for microfluidic platforms and their types such as droplet, centrifugal, and digital microfluidics. Finally, we enumerate some challenges and future directions in microfluidic SERS. Overall, this article showcases the potential and versatility of microfluidic SERS in overcoming the inherent issues in the SERS technique and also discusses the advantage of adding SERS to the arsenal of microfluidics.
随着纳米科学和纳米技术的不断发展，表面增强拉曼光谱（SERS）等分析技术能够提供各种超低浓度分析物分子的结构和化学信息。尽管该技术在各个领域都取得了重大进展，但 SERS 测量的重现性和对小分子的灵敏度仍然是巨大的挑战。在这方面，微流控表面增强拉曼光谱（MF-SERS）正逐渐成为分析化学家工具箱的一部分。本文综述了 MF-SERS 如何成为分析化学中的有力工具。我们批判性地介绍了用于微流控器件的 SERS 基底的发展，以及这些基底在微流道中如何提高 SERS 灵敏度、重现性和检测限。然后，我们介绍了微流控平台的构建材料及其类型，如液滴、离心和数字微流控。最后，我们列举了微流控 SERS 中的一些挑战和未来方向。总的来说，本文展示了微流控 SERS 在克服 SERS 技术固有问题方面的潜力和多功能性，并讨论了将 SERS 技术加入微流控技术的优势。
