Research Center for Analytical Sciences, Northeastern University, Shenyang, 110819, PR China; Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, 110122, PR China.
Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, 110122, PR China.
Biosens Bioelectron. 2022 Feb 15;198:113822. doi: 10.1016/j.bios.2021.113822. Epub 2021 Nov 20.
Raman spectroscopy can realize qualitative and quantitative characterization, and surface-enhanced Raman spectroscopy (SERS) can further enhance its detection sensitivity. In combination with droplet microfluidics, some significant but insurmountable limitations of SERS and Raman spectroscopy can be overcome to some extent, thus improving their detection capability and extending their application. During the past decade, these systems have constantly developed and demonstrated a great potential in more applications, but there is no new review systematically summarizing the droplet microfluidics-based Raman and SERS analysis system since the first related review was published in 2011. Thus, there is a great need for a new review to summarize the advances. In this review, we focus on droplet microfluidics-based Raman and SERS analysis, and summarize two mainstream research directions on this topic up to now. The one is SERS or Raman detection in the moving droplet microreactors, including analysis of molecules, single cells and chemical reaction processes. The other one is SERS active microparticle fabrication via microfluidic droplet templates covering polymer matrix and photonic crystal microparticles. We also comment on the advantages, disadvantage and correlation resolution of droplet microfluidics for SERS or Raman. Finally, we summarize these systems and illustrate our perspectives for future research directions in this field.
拉曼光谱可以实现定性和定量表征，表面增强拉曼光谱（SERS）可以进一步提高其检测灵敏度。与液滴微流控技术相结合，可以在一定程度上克服 SERS 和拉曼光谱的一些显著但不可逾越的限制，从而提高它们的检测能力并扩展其应用。在过去的十年中，这些系统不断发展，并在更多的应用中展示了巨大的潜力，但自 2011 年首次发表相关综述以来，尚无新的综述系统地总结基于液滴微流控的拉曼和 SERS 分析系统。因此，非常有必要进行新的综述来总结进展。在这篇综述中，我们专注于基于液滴微流控的拉曼和 SERS 分析，并总结了迄今为止这一主题的两个主流研究方向。一个是在移动液滴微反应器中的 SERS 或拉曼检测，包括对分子、单细胞和化学反应过程的分析。另一个是通过液滴模板制造用于 SERS 的聚合物基质和光子晶体微珠。我们还对液滴微流控在 SERS 或拉曼方面的优缺点和相关分辨率进行了评论。最后，我们对这些系统进行了总结，并对该领域未来的研究方向提出了展望。
