Zarghami Moghaddam Parastoo, Mohammadi Ameneh, Alesheikh Paiman, Feyzi Peyman, Haghbin Ali, Mollazadeh Samaneh, Sabeti Zahra, Nakhlband Ailar, Kasaian Jamal
North Khorasan University of Medical Sciences, Natural Products and Medicinal Plants Research Center, Bojnurd, Iran.
Mashhad University of Medical Sciences School of Pharmacy, Department of Microbiology and Virology, Mashhad, Iran.
Turk J Pharm Sci. 2021 Feb 25;18(1):10-16. doi: 10.4274/tjps.galenos.2019.59320.
, which belongs to the Capparidaceae family, and has been used in folk medicine for a long time. Our research aims to measure the antioxidant, antibacterial, and antifungal activities of .
Various solvents, such as ethyl acetate, methanol, and dichloromethane, were used to extract different plant parts. Antibacterial and antifungal activities were assayed by disk and well diffusion methods, and the antioxidant activity was screened by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing ability of plasma methods.
Results showed that Gram-negative bacteria and fungus were resistant to various plant extracts. Against all Gram-positive bacteria tested, ' flower extract had the highest inhibition effects. Also, the most sensitive bacterium was , which had an 18-mm inhibition zone. Due to the solvent's physical and chemical properties, different extracts exhibited various antioxidant activities in the antioxidant activity assay. To some extent, methanol extract of leaves showed the highest DPPH radical scavenging activity at various concentrations that ranged from 5 to 160 mg.mL. The methanol extract of flower was observed to have the highest level of phenolics among all tested extracts.
This study demonstrates that different extracts from various parts are different in their properties, therefore, a proper solvent should be used to extract maximum amounts of antioxidant and antibacterial components from a typical plant material.
[植物名称]属于白花菜科，长期以来一直用于民间医学。我们的研究旨在测定[植物名称]的抗氧化、抗菌和抗真菌活性。
使用各种溶剂，如乙酸乙酯、甲醇和二氯甲烷，提取植物的不同部位。采用纸片扩散法和打孔扩散法测定抗菌和抗真菌活性，并通过2,2-二苯基-1-苦基肼自由基（DPPH）法和血浆铁还原能力法筛选抗氧化活性。
结果表明，革兰氏阴性菌和真菌对各种植物提取物具有抗性。对于所有测试的革兰氏阳性菌，[植物名称]花提取物具有最高的抑制作用。此外，最敏感的细菌是[细菌名称]，其抑菌圈为18毫米。由于溶剂的物理和化学性质，不同的[植物名称]提取物在抗氧化活性测定中表现出不同的抗氧化活性。在一定程度上，叶的甲醇提取物在5至160毫克·毫升的不同浓度下表现出最高的DPPH自由基清除活性。在所有测试提取物中，花的甲醇提取物的酚类含量最高。
本研究表明，[植物名称]不同部位的不同提取物性质不同，因此，应使用合适的溶剂从典型植物材料中提取最大量的抗氧化和抗菌成分。
