Department of Ophthalmology, School of Medicine, Collegium Medicum, University of Warmia and Mazury, Olsztyn, Poland.
Regional Specialist Hospital of Olsztyn, Olsztyn, Poland.
Eur J Ophthalmol. 2021 Sep;31(5):2245-2251. doi: 10.1177/1120672121998922. Epub 2021 Feb 25.
Tear fluid, composed of lipid, aqueous, and mucin layers, contains electrolytes, water, proteins, peptides, and glycoproteins. Its components may serve as diagnostic indicators of local and systemic diseases. The aim of the study was to conduct literature review in order to identify the current methods of tear collection. The most commonly used method which was relatively easy to perform and allowed to obtain sufficient tear volume for further chemical and physical analysis was selected through PubMed database search for the following keywords: tear sampling, human tears, chemical analysis of tears, physical tear analysis, animal tear sampling. Final criteria of articles selection were: human tears, tear sample collection, chemical and physical analysis of tears. Time of publication of the articles not older than 1995. The analysis of 70 articles revealed that the most common tear fluid collection methods are Schirmer tear strips and capillary tubes. Thus, we recommend the use of Schirmer strips and microcapillary tubes as the cheapest and easiest methods for sampling of tear fluid for further chemical analysis.
泪液由脂质、水相和黏蛋白层组成，包含电解质、水、蛋白质、肽和糖蛋白。其成分可作为局部和全身疾病的诊断指标。本研究旨在进行文献回顾，以确定目前的泪液采集方法。通过对以下关键词在 PubMed 数据库中的搜索，选择了最常用、相对容易操作且能获得足够的泪液量进行进一步的化学和物理分析的方法：泪液采样、人泪、泪液的化学分析、泪液的物理分析、动物泪液采样。文章选择的最终标准为：人泪、泪液样本采集、泪液的化学和物理分析。文章的出版时间不早于 1995 年。对 70 篇文章的分析表明，最常见的泪液采集方法是 Schirmer 泪液试纸和毛细管。因此，我们建议使用 Schirmer 试纸和微毛细管作为最便宜、最容易的方法，用于进一步的化学分析采样泪液。
