Department of Physiology, Tanta University, Tanta, Egypt.
Department of Pharmacology, Tanta University, Tanta, Egypt.
Arch Physiol Biochem. 2023 Dec;129(4):933-942. doi: 10.1080/13813455.2021.1887265. Epub 2021 Feb 25.
This study declared effect of spexin (SPX) on renal dysfunction in obese rats and its potential mitigating mechanisms which could mediated via galanin receptor-2 (GALR-2). Thirty two 32 Wistar male rats were arranged into four groups: control, high fat/fructose diet (HFFD), HFFD + SPX and HFFD + M871 (galanin receptor 2 antagonist)+SPX. At the termination of the experiment, urine volume, body mass index, Lee index and mean arterial blood pressure were assessed. Renal function was evaluated. Lipid profile, fasting glucose, insulin, insulin resistance and SPX levels were estimated. Also, renal histopathological, immunohistochemical and relative gene expression of renal tissue were done. Also, renal protein carbonyl, reduced glutathione, interferon gamma, monocyte chemoattractant protein-1, interleukin-10 and hydroxyproline were determined.Our results explored that SPX treatment prominently mitigated the metabolic changes and renal dysfunction induced by HFFD via GALR-2. SPX improved insulin resistance, dyslipidemia, renal oxidative stress, inflammation, apoptosis, and fibrosis. So, SPX can be considered as prospective therapeutic agent for treating renal dysfunction.
本研究旨在探讨神经肽 Spexin（SPX）对肥胖大鼠肾功能障碍的影响及其潜在的缓解机制，该机制可能通过甘丙肽受体-2（GALR-2）介导。将 32 只 32 周龄雄性 Wistar 大鼠分为四组：对照组、高脂肪/果糖饮食（HFFD）组、HFFD+SPX 组和 HFFD+M871（甘丙肽受体 2 拮抗剂）+SPX 组。在实验结束时，评估了尿量、体重指数、Lee 指数和平均动脉血压。评估了肾功能。检测了血脂谱、空腹血糖、胰岛素、胰岛素抵抗和 SPX 水平。此外，还进行了肾脏组织的组织病理学、免疫组织化学和相对基因表达检测。此外，还测定了肾脏蛋白羰基、还原型谷胱甘肽、干扰素 γ、单核细胞趋化蛋白-1、白细胞介素-10 和羟脯氨酸。我们的研究结果表明，SPX 通过 GALR-2 治疗可显著减轻 HFFD 引起的代谢变化和肾功能障碍。SPX 改善了胰岛素抵抗、血脂异常、肾脏氧化应激、炎症、细胞凋亡和纤维化。因此，SPX 可以被认为是治疗肾功能障碍的有前途的治疗剂。
