阴道微生态、血清miR-18a和PD-L1对人乳头瘤病毒阳性宫颈癌的诊断价值
Diagnostic Value of Vaginal Microecology, Serum miR-18a, and PD-L1 for Identifying HPV-Positive Cervical Cancer.
作者信息
Zhang Yumei, Qiu Sujuan, Guo Yueli, Zhang Jiaqin, Wu Xiaoqing, Hong Guolin
机构信息
Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, 117892The First Affiliated Hospital of Xiamen University, Xiamen, Fujian Province, China.
Department of Laboratory Medicine, 117892Xinglin Branch of the First Affiliated Hospital of Xiamen University, Xiamen, Fujian Province, China.
出版信息
Technol Cancer Res Treat. 2021 Jan-Dec;20:1533033821995281. doi: 10.1177/1533033821995281.
OBJECTIVE
We aimed to investigate the diagnostic value of the vaginal microecology, serum miR-18a, and programmed death ligand-1 (PD-L1) for human papillomavirus (HPV)-positive cervical cancer.
METHODS
Eighty-four patients with HPV-positive cervical cancer were assigned to the observation group, 107 HPV-positive patients without cervical cancer were assigned to the positive group, and 191 healthy women were assigned to the control group. Vaginal microecology and serum levels of miR-18a and PD-L1 on the surface of CD4 and CD8 T cells were compared among the 3 groups. The observation group was further divided into subgroups according to patients' characteristics for comparison. The diagnostic value of miR-18a and PD-L1 for HPV-positive cervical cancer was investigated.
RESULTS
Women in the control group had better vaginal microecology and lower levels of miR-18a and PD-L1 than those in the observation and the positive groups (all P < 0.05). Compared with the positive group, the observation group had similar vaginal microecology (all P > 0.05) but higher levels of miR-18a and PD-L1 (all P < 0.05). Moreover, the patients at stage III had higher levels of miR-18a and PD-L1 than those at stage I and II (all P < 0.05). The values of area under the curve for miR-18a and PD-L1 in the diagnosis of HPV-positive cervical cancer were over 0.8 (all P < 0.001).
CONCLUSION
Patients with HPV-positive cervical cancer have vaginal microbial dysbiosis and high serum levels of miR-18a and PD-L1. miR-18a and PD-L1 have diagnostic value for identifying HPV-positive cervical cancer.
目的
我们旨在研究阴道微生态、血清miR-18a和程序性死亡配体1（PD-L1）对人乳头瘤病毒（HPV）阳性宫颈癌的诊断价值。
方法
84例HPV阳性宫颈癌患者被分配至观察组，107例HPV阳性但无宫颈癌的患者被分配至阳性组，191例健康女性被分配至对照组。比较3组患者的阴道微生态、miR-18a血清水平以及CD4和CD8 T细胞表面的PD-L1水平。观察组根据患者特征进一步分为亚组进行比较。研究miR-18a和PD-L1对HPV阳性宫颈癌的诊断价值。
结果
与观察组和阳性组相比，对照组女性的阴道微生态更好，miR-18a和PD-L1水平更低（所有P<0.05）。与阳性组相比，观察组的阴道微生态相似（所有P>0.05），但miR-18a和PD-L1水平更高（所有P<0.05）。此外，III期患者的miR-18a和PD-L1水平高于I期和II期患者（所有P<0.05）。miR-18a和PD-L1诊断HPV阳性宫颈癌的曲线下面积值均超过0.8（所有P<0.001）。
结论
HPV阳性宫颈癌患者存在阴道微生物群落失调，血清miR-18a和PD-L1水平较高。miR-18a和PD-L1对识别HPV阳性宫颈癌具有诊断价值。
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