State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.
School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Science. 2021 Feb 26;371(6532). doi: 10.1126/science.abc4346.
Organ homeostasis is orchestrated by time- and spatially restricted cell proliferation. Studies identifying cells with superior proliferative capacities often rely on the lineage tracing of a subset of cell populations, which introduces a potential selective bias. In this work, we developed a genetic system [proliferation tracer (ProTracer)] by incorporating dual recombinases to seamlessly record the proliferation events of entire cell populations over time in multiple organs. In the mouse liver, ProTracer revealed more hepatocyte proliferation in distinct zones during liver homeostasis, injury repair, and regrowth. Clonal analysis showed that most of the hepatocytes labeled by ProTracer had undergone cell division. By genetically recording proliferation events of entire cell populations, ProTracer enables the unbiased detection of specific cellular compartments with enhanced regenerative capacities.
器官稳态由时间和空间限制的细胞增殖来调控。识别具有优越增殖能力的细胞的研究通常依赖于细胞群体的亚群的谱系追踪，这引入了潜在的选择性偏差。在这项工作中，我们开发了一种遗传系统[增殖示踪剂（ProTracer）]，通过整合双重组酶，在多个器官中随时间无缝记录整个细胞群体的增殖事件。在小鼠肝脏中，ProTracer 在肝脏稳态、损伤修复和再生过程中揭示了不同区域的更多肝细胞增殖。克隆分析表明，ProTracer 标记的大多数肝细胞都经历了细胞分裂。通过遗传记录整个细胞群体的增殖事件，ProTracer 能够无偏地检测具有增强再生能力的特定细胞区室。
