Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Laboratório de Sanidade Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil.
Sci Rep. 2021 Feb 25;11(1):4724. doi: 10.1038/s41598-021-83993-5.
Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen associated with a wide variety of infections in humans. The ability of MRSA to infect companion animals has gained increasing attention in the scientific literature. In this study, 334 dogs were screened for MRSA in two cities located in Rio de Janeiro State. The prevalence of MRSA in dogs was 2.7%. Genotyping revealed isolates from sequence types (ST) 1, 5, 30, and 239 either colonizing or infecting dogs. The genome of the canine ST5 MRSA (strain SA112) was compared with ST5 MRSA from humans-the main lineage found in Rio de Janeiro hospitals-to gain insights in the origin of this dog isolate. Phylogenetic analysis situated the canine genome and human strain CR14-035 in the same clade. Comparative genomics revealed similar virulence profiles for SA112 and CR14-035. Both genomes carry S. aureus genomic islands νSAα, νSAβ, and νSAγ. The virulence potential of the canine and human strains was similar in a Caenorhabditis elegans model. Together, these results suggest a potential of canine MRSA to infect humans and vice versa. The circulation in community settings of a MRSA lineage commonly found in hospitals is an additional challenge for public health surveillance authorities.
耐甲氧西林金黄色葡萄球菌（MRSA）是一种与人类多种感染相关的重要病原体。MRSA 感染伴侣动物的能力在科学文献中受到越来越多的关注。在这项研究中，在位于里约热内卢州的两个城市中对 334 只狗进行了 MRSA 筛查。狗中 MRSA 的流行率为 2.7%。基因分型显示来自序列类型（ST）1、5、30 和 239 的分离株定植或感染了狗。对犬 ST5-MRSA（SA112 株）的基因组与来自人类的 ST5-MRSA（里约热内卢医院中主要的谱系）进行了比较，以了解该犬分离株的起源。系统发育分析将犬基因组和人类菌株 CR14-035 置于同一分支中。比较基因组学显示 SA112 和 CR14-035 具有相似的毒力特征。两个基因组均携带金黄色葡萄球菌基因组岛 νSAα、νSAβ 和 νSAγ。在秀丽隐杆线虫模型中，犬和人源菌株的毒力潜力相似。这些结果表明，犬源 MRSA 具有感染人类的潜力，反之亦然。在社区环境中循环的一种通常在医院中发现的 MRSA 谱系是公共卫生监测当局的另一个挑战。
