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液泡与线粒体补丁（vCLAMP）蛋白Mcp1参与维持线粒体功能及线粒体自噬

The Vacuole and Mitochondria Patch (vCLAMP) Protein Mcp1 Is Involved in Maintenance of Mitochondrial Function and Mitophagy in .

作者信息

Mao Xiaolong, Yang Li, Fan Yiming, Wang Jiazhen, Cui Dongkai, Yu Dixiong, Yu Qilin, Li Mingchun

机构信息

Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin, China.

出版信息

Front Microbiol. 2021 Feb 4;12:633380. doi: 10.3389/fmicb.2021.633380. eCollection 2021.

DOI:10.3389/fmicb.2021.633380
PMID:33633712
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7902011/
Abstract

The vacuole and mitochondria patches (vCLAMPs) are novel membrane contact sites in yeast. However, their role in autophagy has not been elucidated so far. In this article, the role of Mcp1, one core component of vCLAMP, in mitophagy of was investigated. Deletion of led to abnormal accumulation of enlarged mitochondria and attenuated stability of mitochondrial DNA (mtDNA) in when cultured in non-fermentable carbon sources. Furthermore, the Δ/Δ mutant exhibited defective growth and degradation of Csp37-GFP. These results indicate that Mcp1 plays a crucial role in mitophagy and maintenance of mitochondrial functions under the non-fermentable condition. Interestingly, this deletion had no impact on degradation of Atg8 (the macroautophagy reporter) and Lap41 (the cytoplasm-to-vacuole targeting pathway marker) under SD-N medium. Moreover, deletion of inhibited filamentous growth and impaired virulence of the pathogen. This study provides an insight to vCLAMPs in cellular functions and pathogenicity in .

摘要

液泡与线粒体斑块（vCLAMPs）是酵母中新型的膜接触位点。然而，它们在自噬中的作用迄今尚未阐明。在本文中，研究了vCLAMP的一个核心组分Mcp1在[具体对象未明确，原文此处有缺失]线粒体自噬中的作用。当在非发酵性碳源中培养时，[具体对象未明确，原文此处有缺失]中[具体基因未明确，原文此处有缺失]的缺失导致线粒体肿大异常积累以及线粒体DNA（mtDNA）稳定性减弱。此外，Δ/Δ突变体表现出Csp37 - GFP生长和降解缺陷。这些结果表明，在非发酵条件下，Mcp1在线粒体自噬及线粒体功能维持中起关键作用。有趣的是，这种缺失对SD - N培养基下Atg8（巨自噬报告基因）和Lap41（细胞质到液泡靶向途径标记物）的降解没有影响。此外，[具体基因未明确，原文此处有缺失]的缺失抑制了病原体的丝状生长并损害了其毒力。本研究为vCLAMPs在[具体对象未明确，原文此处有缺失]的细胞功能和致病性方面提供了见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/97d8e3c9afc7/fmicb-12-633380-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/19756a0f9f43/fmicb-12-633380-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/b3cf9453ff16/fmicb-12-633380-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/4052a04360a8/fmicb-12-633380-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/dd48f2584076/fmicb-12-633380-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/2bddd3e2943f/fmicb-12-633380-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/f4f7417dd685/fmicb-12-633380-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/7c64c58e80dd/fmicb-12-633380-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/3516efb0c293/fmicb-12-633380-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/97d8e3c9afc7/fmicb-12-633380-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/19756a0f9f43/fmicb-12-633380-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/b3cf9453ff16/fmicb-12-633380-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/4052a04360a8/fmicb-12-633380-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/dd48f2584076/fmicb-12-633380-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/2bddd3e2943f/fmicb-12-633380-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/f4f7417dd685/fmicb-12-633380-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/7c64c58e80dd/fmicb-12-633380-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/3516efb0c293/fmicb-12-633380-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc1d/7902011/97d8e3c9afc7/fmicb-12-633380-g009.jpg

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