Wang Xinwei, An Yaqi, Xu Pan, Xiao Jianwei
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China.
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.
Front Plant Sci. 2021 Feb 9;12:627501. doi: 10.3389/fpls.2021.627501. eCollection 2021.
The pentatricopeptide repeat (PPR) proteins constitute one of the largest nuclear-encoded protein families in higher plants, with over 400 members in most sequenced plant species. The molecular functions of these proteins and their physiological roles during plant growth and development have been widely studied. Generally, there is mounting evidence that PPR proteins are involved in the post-transcriptional regulation of chloroplast and/or mitochondrial genes, including RNA maturation, editing, intron splicing, transcripts' stabilization, and translation initiation. The cooperative action of RNA metabolism has profound effects on the biogenesis and functioning of both chloroplasts and mitochondria and, consequently, on the photosynthesis, respiration, and development of plants and their environmental responses. In this review, we summarize the latest research on PPR proteins, specifically how they might function in the chloroplast, by documenting their mechanism of molecular function, their corresponding RNA targets, and their specific effects upon chloroplast biogenesis and host organisms.
五肽重复序列（PPR）蛋白是高等植物中最大的核编码蛋白家族之一，在大多数已测序的植物物种中成员超过400个。这些蛋白的分子功能及其在植物生长发育过程中的生理作用已得到广泛研究。一般来说，越来越多的证据表明PPR蛋白参与叶绿体和/或线粒体基因的转录后调控，包括RNA成熟、编辑、内含子剪接、转录本稳定以及翻译起始。RNA代谢的协同作用对叶绿体和线粒体的生物发生及功能有着深远影响，进而影响植物的光合作用、呼吸作用、发育及其对环境的响应。在本综述中，我们通过记录PPR蛋白的分子功能机制、相应的RNA靶标以及它们对叶绿体生物发生和宿主生物体的特定影响，总结了关于PPR蛋白的最新研究，特别是它们在叶绿体中的作用方式。
