没食子酸对多柔比星诱导的心脏损伤 Wistar 白化大鼠的心脏保护作用。
Cardioprotective Effect of Pistacia vera L. (Green Pistachio) Hull Extract in Wistar Albino Rats with Doxorubicin-Induced Cardiac Damage.
机构信息
Department of Cardiovascular Surgery, Faculty of Medicine, Harran University, Şanlıurfa, Türkiye.
Department of Medical Biochemistry, Faculty of Medicine, Harran University, Şanlıurfa, Türkiye.
出版信息
Anatol J Cardiol. 2023 Mar;27(3):135-145. doi: 10.14744/AnatolJCardiol.2022.2452.
BACKGROUND
Pistacia vera L. (green pistachio) has been shown to increase antioxidant capacity and protect against cardiovascular diseases and cancer. This study investigated the protective effect of the Pistacia vera L. hull in rats with experimental cardiac damage induced by doxorubicin.
METHODS
Sixty adult Wistar albino rats were randomly divided into 5 groups (n = 12). Sham, doxorubicin, doxorubicin + Pistacia vera L. extract 50 mg/kg, doxorubicin + Pistacia vera L. extract 100 mg/kg, and Pistacia vera L. extract 100 mg/kg. Biochemistry parameters, total antioxidant status, total oxidant status, oxidative stress index, 8-hydroxydeoxy guanosine, and caspase 3/7 values were measured in serum samples. Excised heart tissues were examined histopathologically.
RESULTS
The groups were statistically significantly different in 8hydroxydeoxy guanosine, caspase 3/7, total antioxidant status, total oxidant status, oxidative stress index, and basal biochemical parameter values (P <.05, P <.001). In group II, 8-hydroxydeoxy guanosine, caspase 3/7, and total oxidant status values increased while the total antioxidant status value decreased (P <.001). In the treatment groups (group III and group IV), 8-hydroxydeoxy guano sine and caspase 3/7 values decreased compared to group II (P < .001). While total oxidant status and oxidative stress index values decreased in the treatment groups, total antioxidant status values increased (P <.001). The histopathological examination of the heart revealed fewer areas of focal necrosis in the treatment groups compared to group II.
CONCLUSION
In this study, the cardioprotective effect of Pistacia vera L. hull extract was investigated in vivo. It was shown that Pistacia vera L. hull extract reduced apoptosis and deoxyribonucleic acid damage in the face of cardiac damage and had antioxidant activity. Future studies will increase our knowledge on this subject.
背景
已证实开心果（绿开心果）能够提高抗氧化能力，预防心血管疾病和癌症。本研究旨在探讨在阿霉素诱导的实验性心肌损伤大鼠中，开心果壳提取物的保护作用。
方法
将 60 只成年 Wistar 白化大鼠随机分为 5 组（n=12）：假手术组、阿霉素组、阿霉素+50mg/kg 开心果壳提取物组、阿霉素+100mg/kg 开心果壳提取物组和 100mg/kg 开心果壳提取物组。测量血清样本中的生化参数、总抗氧化状态、总氧化状态、氧化应激指数、8-羟基脱氧鸟苷和 caspase 3/7 值。检查切除的心脏组织的组织病理学变化。
结果
各组在 8-羟基脱氧鸟苷、caspase 3/7、总抗氧化状态、总氧化状态、氧化应激指数和基础生化参数值方面均有统计学显著差异（P<.05，P<.001）。在第 2 组中，8-羟基脱氧鸟苷、caspase 3/7 和总氧化状态值增加，而总抗氧化状态值降低（P<.001）。在治疗组（第 3 组和第 4 组）中，8-羟基脱氧鸟苷和 caspase 3/7 值与第 2 组相比降低（P<.001）。治疗组的总氧化状态和氧化应激指数值降低，而总抗氧化状态值升高（P<.001）。与第 2 组相比，治疗组的心脏组织学检查显示局灶性坏死区域较少。
结论
在本研究中，体内研究了开心果壳提取物的心脏保护作用。结果表明，开心果壳提取物减少了心脏损伤时的细胞凋亡和脱氧核糖核酸损伤，并具有抗氧化活性。未来的研究将增加我们对此主题的了解。
Zotero 插件