Peñas María M, Rust Brian, Larraya Luis M, Ramírez Lucía, Pisabarro Antonio G
Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona, Spain.
Appl Environ Microbiol. 2002 Aug;68(8):3891-8. doi: 10.1128/AEM.68.8.3891-3898.2002.
Three different hydrophobins (Vmh1, Vmh2, and Vmh3) were isolated from monokaryotic and dikaryotic vegetative cultures of the edible fungus Pleurotus ostreatus. Their corresponding genes have a number of introns different from those of other P. ostreatus hydrophobins previously described. Two genes (vmh1 and vmh2) were expressed only at the vegetative stage, whereas vmh3 expression was also found in the fruit bodies. Furthermore, the expression of the three hydrophobins varied significantly with culture time and nutritional conditions. The three genes were mapped in the genomic linkage map of P. ostreatus, and evidence is presented for the allelic nature of vmh2 and POH3 and for the different locations of the genes coding for the glycosylated hydrophobins Vmh3 and POH2. The glycosylated nature of Vmh3 and its expression during vegetative growth and in fruit bodies suggest that it should play a role in development similar to that proposed for SC3 in Schizophyllum commune.
从食用菌平菇的单核和双核营养菌丝体培养物中分离出三种不同的疏水蛋白（Vmh1、Vmh2和Vmh3）。它们相应的基因具有一些内含子，与先前描述的其他平菇疏水蛋白的内含子不同。两个基因（vmh1和vmh2）仅在营养阶段表达，而vmh3在子实体中也有表达。此外，三种疏水蛋白的表达随培养时间和营养条件的变化而显著不同。这三个基因被定位在平菇的基因组连锁图谱中，并且有证据表明vmh2和POH3的等位基因性质，以及编码糖基化疏水蛋白Vmh3和POH2的基因的不同位置。Vmh3的糖基化性质及其在营养生长和子实体中的表达表明，它在发育过程中应发挥与裂褶菌中的SC3类似的作用。
