Yperman Klaas, Wang Jie, Eeckhout Dominique, Winkler Joanna, Vu Lam Dai, Vandorpe Michael, Grones Peter, Mylle Evelien, Kraus Michael, Merceron Romain, Nolf Jonah, Mor Eliana, De Bruyn Pieter, Loris Remy, Potocký Martin, Savvides Savvas N, De Rybel Bert, De Jaeger Geert, Van Damme Daniël, Pleskot Roman
Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium.
VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium.
Sci Adv. 2021 Feb 26;7(9). doi: 10.1126/sciadv.abe7999. Print 2021 Feb.
Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as an endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET [TPLATE complex (TPC)] using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generate fresh insight into the differences between the hexameric TSET in and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.
真核细胞依靠内吞作用来调节其质膜蛋白质组和脂质组。除真菌和动物外，大多数真核生物类群都保留了进化上古老的TSET复合体作为内吞调节因子。与其他外被体复合体不同，目前缺乏对TSET的结构认识。在这里，我们使用综合结构方法揭示了植物TSET [TPLATE复合体（TPC）]的分子结构。我们确定了特定TSET亚基在复合体组装和膜相互作用中的关键作用。因此，我们的数据为藻类中的六聚体TSET和植物中的八聚体TPC之间的差异提供了新的见解。这种古老衔接子复合体的结构解析填补了外被体拼图中缺失的一块，并极大地推进了我们对内吞作用过程的功能以及进化认识。
