Institut für Medizinische Physik und Biophysik, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
Institut für Medizinische Physik und Biophysik, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany; Freie Universität Berlin, Research Centre for Electron Microscopy, Fabeckstr. 36a, 14195 Berlin, Germany.
Mol Cell. 2021 Mar 18;81(6):1200-1215.e9. doi: 10.1016/j.molcel.2021.02.006. Epub 2021 Feb 26.
Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.
核糖体生物发生是一个基本的多步骤细胞过程，最终形成核糖体亚基，其产生和修饰受到许多生物发生因子的调节。在这项研究中，我们通过从具有生物发生因子 ObgE 的大肠杆菌菌株中分离出真正的前 50S 亚基来分析生理原核核糖体生物发生，该 ObgE 在其天然基因座上被亲和标记。我们的综合结构方法揭示了一个由 YjgA、RluD、RsfS 和 ObgE 组成的相互作用生物发生因子网络，存在于未成熟的前 50S 亚基上。此外，我们的研究提供了关于 GTP 酶 ObgE 如何与其他生物发生因子协同作用促进 50S 功能核心成熟的机制见解，并揭示了原核生物和真核生物之间核糖体生物发生的保守和不同的进化特征。
