Moremi Nyambura, Silago Vitus, Mselewa Erick G, Chifwaguzi Ashery P, Mirambo Mariam M, Mushi Martha F, Matemba Lucas, Seni Jeremiah, Mshana Stephen E
Quality Assurance & Training Centre, National Health Laboratory, P. O. Box 9083, Dar Es Salaam, Tanzania.
Department of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences-Bugando, P. O. Box 1464, Mwanza, Tanzania.
BMC Res Notes. 2021 Feb 27;14(1):77. doi: 10.1186/s13104-021-05495-x.
The objective of this study was to determine the proportion of extended spectrum β-lactamase producing gram-negative bacteria (ESBL-GNB) colonizing patients admitted at Mazimbu hospital and Morogoro Regional hospital, in Morogoro, Tanzania. Rectal colonization with ESBL-GNB increases the risks of developing bacterial infections by extra-intestinal pathogenic ESBL-GNB.
Of the 285 patients investigated, 123 (43.2%) carried ESBL-GNB in their intestines. Five of the 123 ESBL positive patients were colonized with two different bacteria, making a total of 128 ESBL producing isolates. Escherichia coli (n = 95, 74.2%) formed the majority of ESBL isolates. The proportion of CTX-M-1 group genes among ESBL isolates tested was 94.9% (93/98). History of antibiotic use (OR: 1.83, 95% CI: 1.1-3.2, P = 0.03), being on antibiotic treatment (OR: 2.61, 95% CI: 1.5-4.53, P = 0.001), duration of hospital stay (OR: 1.2, 95% CI: 1.1-1.3, P < 0.001) and history of previous admission (OR: 2.24, 95% CI: 1.2-4.1, P = 0.009) independently predicted ESBL-GNB carriage.
本研究的目的是确定在坦桑尼亚莫罗戈罗的马津布医院和莫罗戈罗地区医院住院患者中，产超广谱β-内酰胺酶革兰氏阴性菌（ESBL-GNB）的定植比例。ESBL-GNB在直肠定植会增加肠道外致病性ESBL-GNB引发细菌感染的风险。
在285名接受调查的患者中，123名（43.2%）肠道中携带ESBL-GNB。123名ESBL阳性患者中有5名被两种不同细菌定植，共有128株产ESBL分离株。大肠埃希菌（n = 95，74.2%）是ESBL分离株中的主要菌群。在检测的ESBL分离株中，CTX-M-1组基因的比例为94.9%（93/98）。抗生素使用史（比值比：1.83，95%置信区间：1.1 - 3.2，P = 0.03）、正在接受抗生素治疗（比值比：2.61，95%置信区间：1.5 - 4.53，P = 0.001）、住院时间（比值比：1.2，95%置信区间：1.1 - 1.3，P < 0.001）和既往住院史（比值比：2.24，95%置信区间：1.2 - 4.1，P = 0.009）可独立预测ESBL-GNB携带情况。
