He Haitian, Li Jianhua, Luo Mayao, Wei Qiang
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Department of Urology, Shenzhen Nanshan District Shekou People's Hospital, Shenzhen, Guangdong, China.
J Int Med Res. 2021 Feb;49(2):300060521992215. doi: 10.1177/0300060521992215.
Circular RNAs (circRNAs) are non-coding RNAs with high cancer-specific expression and the potential for regulating tumorigenesis. CircRNA_100395 is expressed at low levels in many cancers and is involved in the regulation of tumor cell proliferation and metastasis. However, its expression and function in prostate cancer remain unclear.
Endogenous expression levels of circRNA_100395 and microRNA-1228 (miR-1228) in prostate cancer tissue samples and cell lines were detected by quantitative reverse transcription-polymerase chain reaction. Cell proliferation, invasion, and migration, cell cycle distribution, and epithelial-mesenchymal transition (EMT) were analyzed in circRNA_100395-overexpressing prostate cancer cells by Cell Counting Kit-8, flow cytometry, Transwell assay, and western blotting, respectively.
CircRNA_100395 expression was downregulated in cancerous prostate tissues relative to adjacent normal tissues. CircRNA_100395 expression was negatively correlated with tumor size, Gleason score, tumor stage, and lymph node metastasis. Moreover, circRNA_100395 overexpression inhibited cell proliferation, altered cell cycle distribution, reduced cell migration and invasion abilities, and suppressed EMT in prostate cancer cells. Moreover, miR-1228 was a direct downstream target of circRNA_100395, and the anti-tumor ability of circRNA_100395 was significantly reversed by miR-1228.
This study identified circRNA_100395 as an anti-tumor circRNA and a potential therapeutic target for prostate cancer.
环状RNA（circRNAs）是具有高癌症特异性表达且有调控肿瘤发生潜力的非编码RNA。CircRNA_100395在多种癌症中低表达，并参与肿瘤细胞增殖和转移的调控。然而，其在前列腺癌中的表达及功能仍不清楚。
通过定量逆转录-聚合酶链反应检测前列腺癌组织样本和细胞系中circRNA_100395和微小RNA-1228（miR-1228）的内源性表达水平。分别采用细胞计数试剂盒-8、流式细胞术、Transwell实验和蛋白质印迹法分析circRNA_100395过表达的前列腺癌细胞的细胞增殖、侵袭、迁移、细胞周期分布及上皮-间质转化（EMT）。
与相邻正常组织相比，circRNA_100395在前列腺癌组织中的表达下调。CircRNA_100395的表达与肿瘤大小、Gleason评分、肿瘤分期及淋巴结转移呈负相关。此外，circRNA_100395过表达抑制前列腺癌细胞增殖，改变细胞周期分布，降低细胞迁移和侵袭能力，并抑制EMT。而且，miR-1228是circRNA_100395的直接下游靶点，miR-1228显著逆转了circRNA_100395的抗肿瘤能力。
本研究确定circRNA_100395为一种抗肿瘤circRNA及前列腺癌的潜在治疗靶点。
