Shen Naian, Zhang Yue, Raza Ali, Chang Liu, Wang Jin-Ye
School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
Mater Sci Eng C Mater Biol Appl. 2021 Mar;122:111900. doi: 10.1016/j.msec.2021.111900. Epub 2021 Jan 23.
In order to prevent thrombosis, reduce intima hyperplasia, and to maintain long-term patency after implantation of an artificial blood vessel, the formation of intact endothelial cells layer on an inner surface of graft is desirable. The present study aimed to improve endothelial cell adhesion by regulating the morphology of the inner surface of artificial blood vessels. Zein fibre membranes with three fibre diameters (small, ~100 nm; medium, ~500 nm; and large, ~1000 nm) were constructed by electrospinning. A flow chamber device was designed to simulate the blood flow environment. The morphology and adhesion of human umbilical vein fusion cells (EA.hy926) on the surface of the fibre membranes were studied under a shear stress of approximately 15 dynes/cm. The results showed that oriented electrospun zein fibre surfaces with both medium- and large-diameter fibres can regulate the morphology of endothelial cells (EA.hy926), which are aligned by the fibre direction. The three fibre membranes improved the adhesion of endothelial cells significantly compared to that on the flat membrane. When the fibre direction was fixed parallel to the fluid direction, the medium-diameter oriented-fibre membrane could significantly improve the ability endothelial cells to resist shear stress, and there was a significant difference at 1, 2 and 4 h time points compared with the shear stress resistance on the small-diameter and large-diameter oriented-fibre membranes. When the fibre direction was perpendicular to the fluid direction, again the medium-diameter oriented-fibre membrane improved the ability of endothelial cells to resist shear stress significantly at 1 and 2 h time points. It was concluded that by changing the diameter and arrangement of electrospun fibres, cell morphology control and shear stress resistance can be achieved.
为了预防血栓形成、减少内膜增生，并在植入人造血管后维持长期通畅，在移植物内表面形成完整的内皮细胞层是理想的。本研究旨在通过调节人造血管内表面的形态来改善内皮细胞的黏附。通过静电纺丝构建了三种纤维直径（小，约100nm；中，约500nm；大，约1000nm）的玉米醇溶蛋白纤维膜。设计了流动腔装置来模拟血流环境。在约15达因/平方厘米的剪切应力下，研究了人脐静脉融合细胞（EA.hy926）在纤维膜表面的形态和黏附情况。结果表明，中直径和大直径纤维的取向静电纺玉米醇溶蛋白纤维表面可调节内皮细胞（EA.hy926）的形态，使其沿纤维方向排列。与平膜相比，三种纤维膜显著提高了内皮细胞的黏附。当纤维方向固定为与流体方向平行时，中直径取向纤维膜可显著提高内皮细胞抵抗剪切应力的能力，在1、2和4小时时间点与小直径和大直径取向纤维膜的抗剪切应力能力相比有显著差异。当纤维方向垂直于流体方向时，同样中直径取向纤维膜在1和2小时时间点显著提高了内皮细胞抵抗剪切应力的能力。得出的结论是，通过改变静电纺纤维的直径和排列，可以实现细胞形态控制和抗剪切应力能力。
