Hanifah Nada, Achmad Yusuf Farid, Humaira Aida, Salasia Siti Isrina Oktavia
Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Vet World. 2021 Jan;14(1):176-181. doi: 10.14202/vetworld.2021.176-181. Epub 2021 Jan 21.
Red ginger (RG) has reportedly been used in folk medicine for the management and prevention of hypertension. One of the hypertension study models in experimental animals is the unilateral ureteral obstruction (UUO). This study aimed at evaluating the effect of RG-extract (RGE) nanoemulsion on UUO-induced hypertension and angiotensin-converting enzyme (ACE) production in rats.
RG was extracted using ethanol, combined with virgin coconut oil, polysorbate 80, and polyethylene glycol 400 to form the oil phase. The particle sizes of RGE nanoemulsions were analyzed using a particle size analyzer. The UUO method was used to induce chronic kidney disease in rats (504 mg/200 g and 360 mg/200 g b/w per oral for 7 days). The systolic and diastolic blood pressure was determined non-invasively in conscious state by tail plethysmography using an automated blood pressure monitor. ACE in serum was measured using enzyme-linked immunosorbent assay.
The RGE nanoemulsions exhibited a particle size of32.8 nm and a polydispersity index (PI) of 0.268, indicating a homogenous nanoemulsion. UUO rats treated with RGE nanoemulsion (360 mg/200 g b/w) experienced a significant decrease in both their systolic blood pressure (p<0.05) from 142±1 mmHg to 107±6 mmHg and their diastolic blood pressure from 106±1 mmHg to 84±4 mmHg. Furthermore, treatment with RGE resulted in a 10.80% decrease in the level of ACE.
The size and the PI of the RGE used in this study suggest a stable and effective distribution of the particle size in the emulsions. RGE nanoemulsions at the dose of 360 mg/200 g bw can be used as potential ACE inhibitors because they were found to decrease the blood pressure of hypertensive UUO rats.
据报道，红姜已被用于民间医学中治疗和预防高血压。实验动物高血压研究模型之一是单侧输尿管梗阻（UUO）。本研究旨在评估红姜提取物（RGE）纳米乳剂对UUO诱导的大鼠高血压及血管紧张素转换酶（ACE）产生的影响。
用乙醇提取红姜，与初榨椰子油、聚山梨酯80和聚乙二醇400混合形成油相。使用粒度分析仪分析RGE纳米乳剂的粒径。采用UUO方法诱导大鼠慢性肾病（口服剂量为504 mg/200 g和360 mg/200 g体重，持续7天）。使用自动血压监测仪通过尾容积描记法在清醒状态下无创测定收缩压和舒张压。采用酶联免疫吸附测定法测量血清中的ACE。
RGE纳米乳剂的粒径为32.8 nm，多分散指数（PI）为0.268，表明为均匀的纳米乳剂。用RGE纳米乳剂（360 mg/200 g体重）治疗的UUO大鼠收缩压从142±1 mmHg显著降至107±6 mmHg（p<0.05），舒张压从106±1 mmHg降至84±4 mmHg。此外，RGE治疗使ACE水平降低了10.80%。
本研究中使用的RGE的粒径和PI表明其在乳剂中粒径分布稳定且有效。剂量为360 mg/200 g体重的RGE纳米乳剂可作为潜在的ACE抑制剂，因为它们能降低高血压UUO大鼠的血压。
