Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3.

OBJECTIVE

This study set out to investigate the effect of on lung cancer progression through targeted regulation of .

METHODS

RT-PCR was employed to detect the expression levels in lung cancer cells and its targeted gene mRNA determined by biological information prediction. MTT, invasion and apoptosis rate tests were employed to detect the proliferation, invasion and apoptosis rate of lung cancer cells over-expressing or those with low expression of and the expression of related proteins.

RESULTS

RT-qPCR results manifested that the level was down-regulated in lung cancer tissues and cells, and the expression increased. The and expression levels were negatively correlated. was correlated with tumor differentiation degree, TNM stage and lymph node metastasis of lung cancer patients. Cell tests confirmed that played a tumor-inhibiting function, including inhibiting proliferation and invasion of lung cancer cells and promoting apoptosis. Bioinformatics prediction and subsequent experiments proved that was the direct target of . Moreover, after the expression in lung cancer cells was knocked down, proliferation and invasion of those cells were inhibited dramatically, the apoptosis rate increased markedly, and the expression levels of pro-apoptosis-related proteins and were up-regulated, and the anti-apoptosis-related protein expression was down-regulated.

CONCLUSION

can inhibit the proliferation and invasion of lung cancer cells and promote their apoptosis by targeting . It can be used as a new potential target for lung cancer treatment.