Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, China.
Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha, China.
Front Immunol. 2021 Feb 12;12:630112. doi: 10.3389/fimmu.2021.630112. eCollection 2021.
Systemic lupus erythematosus (SLE) is a multi-system autoimmune disease characterized by continuous inflammation and the production of autoantibodies. Exosomes, acting as a critical tool for communication between cells, are involved in the pathogenesis of SLE, particularly in inflammation and immune imbalance. In this study, we aimed to extract and confirm the pro-inflammatory effect of serum exosomes in SLE. Then, we attempted to find differentially expressed exosomal microRNAs in the serum of healthy subjects and SLE patients miRNA microarray analysis and validated the target exosomal microRNA, exosomal miR-451a, which expression level decreased in serum of SLE patients by RT-qPCR. Furtherly, we analyzed the correlation between exosomal miR-451a and disease activity, kidney damage and typing, and traditional medicine therapy. Finally, we investigated the intercellular communication role of exosomal miR-451a in SLE by co-culture assay . Taken together, our study demonstrated that downregulated serum exosomal miR-451a expression correlated with SLE disease activity and renal damage as well as its intercellular communication role in SLE which provided potential therapeutic strategies.
系统性红斑狼疮（SLE）是一种多系统自身免疫性疾病，其特征为持续炎症和自身抗体的产生。外泌体作为细胞间通讯的重要工具，参与了 SLE 的发病机制，特别是在炎症和免疫失衡中。在本研究中，我们旨在提取并确认血清外泌体在 SLE 中的促炎作用。然后，我们尝试通过 miRNA 微阵列分析发现健康受试者和 SLE 患者血清中差异表达的外泌体 microRNA，并通过 RT-qPCR 验证了 SLE 患者血清中表达水平降低的靶向外泌体 microRNA，外泌体 miR-451a。进一步，我们分析了外泌体 miR-451a 与疾病活动度、肾脏损伤和分型以及传统医学治疗的相关性。最后，我们通过共培养试验研究了外泌体 miR-451a 在 SLE 中的细胞间通讯作用。综上所述，我们的研究表明，下调的血清外泌体 miR-451a 表达与 SLE 疾病活动度和肾脏损伤以及其在 SLE 中的细胞间通讯作用相关，为提供潜在的治疗策略提供了依据。
