Malekzadeh Kebria Maziar, Salehnia Mojdeh, Zavareh Saeed, Moazzeni Seyyed Saeed
Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran.
Vet Res Forum. 2020 Fall;11(4):377-383. doi: 10.30466/vrf.2018.93471.2255. Epub 2020 Dec 15.
maturation (IVM) of oocytes is widely used in assisted reproduction technologies. The present study aimed to improve the oocyte maturation and its development through enriching the culture media with sodium selenite (SS). Moreover, the effects of SS on the expression of the oocytes apoptosis-related genes were assessed. In this study, male and female NMRI mice were used and after collecting their germinal vesicle (GV) oocytes, they were cultured with SS (experimental group) and without SS (control group). Collected metaphase II oocytes (MII) from the fallopian tube were considered as group. After culture, the oocytes were assessed in terms of nuclear maturation. The MII oocytes were inseminated and the development was examined until the blastocyst stage. Also, oocytes were subjected to the molecular analysis for evaluating the expression of BAX, BCL2, P53, and BAD genes using the real-time RT-PCR. The maturation rate was significantly increased in the SS supplemented group compared to the control one. The developmental rate of the embryos was significantly higher for both of the and SS supplemented groups rather than the control one, however, no significant difference was seen between these rates of the experimental and groups. Real-time RT-PCR did not show any significant differences in the expression of the apoptosis-related genes for all of the studied groups. The p53 gene was not expressed in any of groups. Sodium selenite improved the oocyte developmental competence but did not change the expression of the apoptosis-related genes in MII oocytes.
卵母细胞的体外成熟（IVM）在辅助生殖技术中被广泛应用。本研究旨在通过在培养基中添加亚硒酸钠（SS）来提高卵母细胞的成熟度及其发育能力。此外，还评估了SS对卵母细胞凋亡相关基因表达的影响。在本研究中，使用了雄性和雌性NMRI小鼠，收集其生发泡（GV）期卵母细胞后，将其分为添加SS的实验组和不添加SS的对照组进行培养。将从输卵管收集的中期II期卵母细胞（MII）作为一组。培养后，从核成熟方面评估卵母细胞。对MII期卵母细胞进行授精，并检查其发育至囊胚期。此外，使用实时RT-PCR对卵母细胞进行分子分析，以评估BAX、BCL2、P53和BAD基因的表达。与对照组相比，添加SS的组成熟率显著提高。实验组和添加SS组的胚胎发育率均显著高于对照组，但实验组和该组之间的发育率无显著差异。实时RT-PCR结果显示，所有研究组的凋亡相关基因表达均无显著差异。p53基因在任何组中均未表达。亚硒酸钠提高了卵母细胞的发育能力，但未改变MII期卵母细胞凋亡相关基因的表达。
