Morejon Andy, Norberg Christopher D, De Rosa Massimiliano, Best Thomas M, Jackson Alicia R, Travascio Francesco
Department of Mechanical and Aerospace Engineering, University of Miami, Coral Gables, FL, United States.
Department of Biomedical Engineering, University of Miami, Coral Gables, FL, United States.
Front Bioeng Biotechnol. 2021 Feb 10;8:622552. doi: 10.3389/fbioe.2020.622552. eCollection 2020.
The meniscus is crucial in maintaining knee function and protecting the joint from secondary pathologies, including osteoarthritis. The meniscus has been shown to absorb up to 75% of the total load on the knee joint. Mechanical behavior of meniscal tissue in compression can be predicted by quantifying the mechanical parameters including; aggregate modulus () and Poisson modulus (ν), and the fluid transport parameter: hydraulic permeability (). These parameters are crucial to develop a computational model of the tissue and for the design and development of tissue engineered scaffolds mimicking the native tissue. Hence, the objective of this study was to characterize the mechanical and fluid transport properties of human meniscus and relate them to the tissue composition. Specimens were prepared from the axial and the circumferential anatomical planes of the tissue. Stress relaxation tests yielded the , while finite element modeling was used to curve fit for ν and . Correlations of moduli with water and glycosaminoglycans (GAGs) content were investigated. On average was found to be 0.11 ± 0.078 MPa, ν was 0.32 ± 0.057, and was 2.9 ± 2.27 × 10 mNs. The parameters , ν, and were not found to be statistically different across compression orientation or compression level. Water content of the tissue was 77 ± 3.3% while GAG content was 8.79 ± 1.1%. Interestingly, a weak negative correlation was found between and water content (R ~ 34%) and a positive correlation between and GAG content (R ~ 53%). In conclusion, while no significant differences in transport and compressive properties can be found across sample orientation and compression levels, data trends suggest potential relationships between magnitudes of H and K, and GAG content.
半月板对于维持膝关节功能以及保护关节免受包括骨关节炎在内的继发性病变至关重要。已证明半月板可吸收膝关节总负荷的75%。通过量化包括聚集模量（）和泊松模量（ν）以及流体传输参数：水力渗透率（）在内的力学参数，可以预测半月板组织在压缩时的力学行为。这些参数对于建立组织的计算模型以及设计和开发模仿天然组织的组织工程支架至关重要。因此，本研究的目的是表征人半月板的力学和流体传输特性，并将它们与组织组成相关联。从组织的轴向和周向解剖平面制备标本。应力松弛试验得出了，而有限元建模用于对ν和进行曲线拟合。研究了模量与水和糖胺聚糖（GAGs）含量的相关性。平均而言，发现为0.11±0.078MPa，ν为0.32±0.057，为2.9±2.27×10mNs。未发现、ν和在压缩方向或压缩水平上存在统计学差异。组织的含水量为77±3.3%，而GAG含量为8.79±1.1%。有趣的是，发现与含水量之间存在弱负相关（R34%），与GAG含量之间存在正相关（R53%）。总之，虽然在样品方向和压缩水平上未发现传输和压缩特性的显著差异，但数据趋势表明H和K的大小与GAG含量之间可能存在关系。
