Leek J C, Keen C L, Vogler J B, Golub M S, Hurley L S, Hendrickx A G, Gershwin M E
School of Medicine, University of California, Davis 95616.
Am J Clin Nutr. 1988 May;47(5):889-95. doi: 10.1093/ajcn/47.5.889.
Skeletal maturation was evaluated from ages 1 to 3 y in rhesus monkeys that had been subjected to a diet marginally deficient in zinc (4 micrograms/g Zn) from conception through age 3 y. Skeletal development was assessed at 18, 24, 30, and 36 mo of age and compared with that of controls fed ad libitum. Skeletal maturation was determined by the presence of epiphyseal ossification centers. To evaluate endochondral bone mineralization the appearance of the zone of provisional calcification on the metaphyseal side of the growth plate and the width of the growth plate were observed. Marginal Zn deprivation was associated with delayed skeletal maturation in monkeys up to age 3 y. Defective mineralization of bone was evident in these monkeys up to age 6 mo. Between ages 6 mo and 3 y bone mineralization increased in some of the marginal-Zn monkeys to values that were only slightly below those for control monkeys.
对从受孕到3岁期间摄入锌含量略低（4微克/克锌）饮食的恒河猴，评估其1至3岁时的骨骼成熟情况。在18、24、30和36月龄时评估骨骼发育，并与自由采食的对照组进行比较。通过骨骺骨化中心的存在来确定骨骼成熟度。为评估软骨内骨矿化，观察生长板干骺端一侧临时钙化带的外观和生长板的宽度。锌轻度缺乏与3岁以下猴子的骨骼成熟延迟有关。在这些猴子中，直至6月龄时骨矿化缺陷明显。在6月龄至3岁之间，一些轻度缺锌猴子的骨矿化增加至仅略低于对照猴子的值。
