Wu Mingxuan, Xia Mingyu, Li Wenyan, Li Huawei
ENT Institute and Department of Otorhinolaryngology, Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China.
Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Front Cell Dev Biol. 2021 Feb 12;9:637779. doi: 10.3389/fcell.2021.637779. eCollection 2021.
Genomics studies face specific challenges in the inner ear due to the multiple types and limited amounts of inner ear cells that are arranged in a very delicate structure. However, advances in single-cell sequencing (SCS) technology have made it possible to analyze gene expression variations across different cell types as well as within specific cell groups that were previously considered to be homogeneous. In this review, we summarize recent advances in inner ear research brought about by the use of SCS that have delineated tissue heterogeneity, identified unknown cell subtypes, discovered novel cell markers, and revealed dynamic signaling pathways during development. SCS opens up new avenues for inner ear research, and the potential of the technology is only beginning to be explored.
由于内耳细胞类型多样且数量有限，并且以非常精细的结构排列，基因组学研究在内耳面临着特定的挑战。然而，单细胞测序（SCS）技术的进步使得分析不同细胞类型之间以及先前被认为是同质的特定细胞群内的基因表达变异成为可能。在这篇综述中，我们总结了使用SCS在内耳研究中取得的最新进展，这些进展已经描绘了组织异质性、识别了未知的细胞亚型、发现了新的细胞标志物，并揭示了发育过程中的动态信号通路。SCS为内耳研究开辟了新途径，并且该技术的潜力才刚刚开始被探索。
