Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Laboratory of Lipid Neurochemistry, European Center for Brain Research (CERC), Santa Lucia Foundation IRCCS, 00143 Rome, Italy.
Biomolecules. 2023 Oct 17;13(10):1534. doi: 10.3390/biom13101534.
Advanced genomics, transcriptomics, and epigenomics techniques are providing unprecedented insights into the understanding of the molecular underpinnings of the central nervous system, including the neuro-sensory cochlea of the inner ear. Here, we report for the first time a comprehensive and updated overview of the most advanced omics techniques for the study of nucleic acids and their applications in cochlear research. We describe the available in vitro and in vivo models for hearing research and the principles of genomics, transcriptomics, and epigenomics, alongside their most advanced technologies (like single-cell omics and spatial omics), which allow for the investigation of the molecular events that occur at a single-cell resolution while retaining the spatial information.
先进的基因组学、转录组学和表观基因组学技术为深入理解中枢神经系统（包括内耳的感觉神经耳蜗）的分子基础提供了前所未有的见解。在这里，我们首次全面和更新地概述了研究核酸的最先进的组学技术及其在耳蜗研究中的应用。我们描述了用于听力研究的现有体外和体内模型，以及基因组学、转录组学和表观基因组学的原理，以及它们最先进的技术（如单细胞组学和空间组学），这些技术允许在保留空间信息的同时，以单细胞分辨率研究发生的分子事件。
