Ganushkina Lyudmila, Lukashev Alexander, Patraman Ivan, Razumeyko Vladimir, Shaikevich Elena
Martsinovsky Institute of Medical Parasitology, Tropical and Vector-Borne Diseases, Sechenov First Moscow State Medical University, Moscow, Russia.
Department of Ecology and Zoology Taurida Academia, Vernadsky Cremian Federal University, Simferopol, Republic of Crimea.
J Arthropod Borne Dis. 2020 Sep 30;14(3):270-276. doi: 10.18502/jad.v14i3.4560. eCollection 2020 Sep.
The incidence and area of arbovirus infections is increasing around the world. It is largely linked to the spread of the main arbovirus vectors, invasive mosquito of the genus Previously, it has been reported that reemerged in Russia after a 50-year absence. Moreover, in 2011, was registered in the city of Sochi (South Russia, Black Sea coast) for the first time. In 2013, Asian was found in Sochi for the first time.
Mosquitoes were collected using the following methods: larvae with a dip net, imago on volunteers and using bait traps. The mosquitoes were identified using both morphology and sequencing of the second internal transcribed spacer of the nuclear ribosomal RNA gene cluster.
In August 2016, larvae and imago and a single male of were found on the southern coast of the Crimean Peninsula, where they were not registered before. Newly obtained DNA sequences were registered in GenBank with the accession numbers MF072936 and MF072937.
Detection of invasive mosquito species ( and ) implies the possibility of their area expansion. Intensive surveillance is required at the Crimean Peninsula to evaluate the potential for the introduction of vector-borne diseases.
虫媒病毒感染的发病率和感染区域在全球范围内不断增加。这在很大程度上与主要虫媒病毒传播媒介——伊蚊属入侵蚊子的扩散有关。此前有报道称，在消失50年后于俄罗斯重新出现。此外，2011年，首次在索契市（俄罗斯南部，黑海沿岸）被记录到。2013年，亚洲伊蚊首次在索契被发现。
采用以下方法收集蚊子：用抄网收集幼虫，在志愿者身上收集成虫，并使用诱饵诱捕器。通过形态学和核糖体RNA基因簇第二个内部转录间隔区的测序对蚊子进行鉴定。
2016年8月，在克里米亚半岛南岸发现了伊蚊幼虫、成虫以及一只单独的雄蚊，此前该地区未记录到这些蚊子。新获得的DNA序列已在GenBank注册，登录号为MF072936和MF072937。
入侵蚊种（伊蚊和亚洲伊蚊）的发现意味着它们有扩大分布区域的可能性。需要在克里米亚半岛进行强化监测，以评估引入媒介传播疾病的可能性。
