Soni Vineet, Keswani Kiran, Bhatt Upma, Kumar Deepak, Singh Hanwant
Plant Bioenergetics & Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur 313001, Rajasthan, India.
Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune 411045, Maharashtra, India.
Heliyon. 2021 Feb 3;7(2):e06101. doi: 10.1016/j.heliyon.2021.e06101. eCollection 2021 Feb.
An efficient and reproducible protocol for propagation of has been established for the first time from nodal segments. In order to enhance survival rate under conditions, photosynthetic potential of grown plantlets was also studied through JIP test based analysis of polyphasic OJIP chlorophyll fluorescence OJIP transients, density of active reaction centers, light harvesting efficiency, electron transfer rate, dissipation energy, maximum quantum yield of primary PSII photochemistry and photosynthetic performance index. The best morphogenetic in term of explants response (92.2 %), shoot number (3.43 ± 0.07) and shoot length (4.7 ± 0.31 cm) was obtained on Murashige and Skoog medium supplemented with 0.5 mg l BAP and 1.0 mg l TDZ. The shoots exhibited high frequency rhizogenesis on half strength medium augmented with 2.0 mg l IAA. plantlets developed highest rate of photosynthesis on day 18 after the initiation of rhizogenesis. High survival rate (96.16%) under ex vitro conditions was observed when plantlets having high photosynthetic efficiency (Fv/Fm > 0.75) were subjected to hardening and acclimatization process. Plantlets with reduced photosynthetic performance exhibited low survival rate under natural conditions. The developed protocol will be useful for genetic improvement and multiplication of . The results of this study also show that photosynthetic screening of developed plantlets is highly essential after the rhizogenesis process to achieve higher survival rate under field conditions.
首次从节段建立了一种高效且可重复的[植物名称]繁殖方案。为了提高[特定条件下]的成活率，还通过基于JIP测试的多相OJIP叶绿素荧光OJIP瞬变分析、活性反应中心密度、光捕获效率、电子传递速率、耗散能量、初级PSII光化学的最大量子产率和光合性能指数，研究了生长的小植株的光合潜力。在添加0.5 mg/L BAP和1.0 mg/L TDZ的Murashige和Skoog培养基上，外植体反应（92.2%）、芽数（3.43±0.07）和芽长（4.7±0.31 cm）方面的最佳形态发生效果得以实现。芽在添加2.0 mg/L IAA的半强度培养基上表现出高频生根。[植物名称]小植株在生根开始后第18天光合作用速率最高。当光合效率高（Fv/Fm>0.75）的[植物名称]小植株进行炼苗和驯化过程时，在离体条件下观察到高成活率（96.16%）。光合性能降低的小植株在自然条件下成活率低。所建立的方案将有助于[植物名称]的遗传改良和繁殖。本研究结果还表明，在生根过程后对发育的小植株进行光合筛选对于在田间条件下实现更高的成活率至关重要。
