Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
Novosibirsk State Technical University, Novosibirsk, Russia.
Arch Insect Biochem Physiol. 2021 Apr;106(4):e21776. doi: 10.1002/arch.21776. Epub 2021 Feb 28.
Maternally inherited endosymbiotic bacterium Wolbachia infects Drosophila melanogaster populations worldwide. Its genetic diversity includes several closely related genotypes, which can be attributed to two main genotype groups: wMel and wMelCS. Here, we studied eight D. melanogaster lines carrying the nuclear background of wild type interbred Bi90 line and cytoplasmic backgrounds with or without Wolbachia of different origin, each of which belongs to wMelCS genotype group. We analyzed the effect these seven Wolbachia strains had on the heat stress resistance and dopamine metabolism in D. melanogaster females. Survival under heat stress (38°C, 3 h 30 min) was increased only in the line infected with bacteria of the wMelPlus strain. At the same time, the activity of alkaline phosphatase (an enzyme regulating the pool of dopamine precursor tyrosine) was increased under normal conditions in females infected with all strains under study and retained the response to heat stress typical for the uninfected line. Thus, we found the unique Wolbachia strain that provides an increase of the host stress resistance, and demonstrated that the mechanism of this resistance is not dopamine-mediated.
母系共生细菌沃尔巴克氏体感染了世界各地的黑腹果蝇种群。其遗传多样性包括几个密切相关的基因型，可归因于两个主要的基因型群：wMel 和 wMelCS。在这里，我们研究了携带野生型杂交 Bi90 核背景和不同来源细胞质背景的 8 条黑腹果蝇品系，其中每条品系都属于 wMelCS 基因型群。我们分析了这 7 种沃尔巴克氏体菌株对黑腹果蝇雌性热应激抵抗力和多巴胺代谢的影响。只有感染 wMelPlus 菌株的细菌的品系在热应激（38°C，3 小时 30 分钟）下的存活率增加。同时，在正常条件下，所有研究菌株感染的雌性的碱性磷酸酶（一种调节多巴胺前体酪氨酸库的酶）活性增加，并保留了未感染品系的典型热应激反应。因此，我们发现了一种独特的沃尔巴克氏体菌株，它可以提高宿主的应激抵抗力，并证明这种抵抗力的机制不是多巴胺介导的。
