Jiang Rui-Xu, Shang Feng, Jiang Hong-Bo, Dou Wei, Cernava Tomislav, Wang Jin-Jun
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Microbiol Spectr. 2023 Mar 28;11(2):e0529822. doi: 10.1128/spectrum.05298-22.
Insects are associated with diverse microbial communities that can have substantial effects on hosts. Here, we characterized the bacterial communities in the Asian citrus psyllid (ACP), Diaphorina citri (Hemiptera: Psyllidae), a major vector of the devastating pathogen " Liberibacter asiaticus," which causes citrus Huanglongbing (HLB). In total, 256 ACP individuals across 15 field sites and one laboratory population in China were sequenced. The results showed that the bacterial community diversity was the highest in the Guilin population (average Shannon index, 1.27), and the highest value for richness was found in the Chenzhou population (average Chao1 index, 298). The bacterial community structures of the field-collected populations were significantly different, and all of them harbored , which was assigned to strain ST-173. Structural equation models revealed that the dominant strain had a significantly negative correlation with the annual mean temperature. In addition, the results obtained with populations infected with " Liberibacter asiaticus" indicated that in total, 140 bacteria could be involved in interactions with this bacterium. The ACP field populations harbored a more diverse bacterial community than the laboratory population, and the relative occurrences of some symbionts differed significantly. However, the bacterial community of the ACP laboratory colony was connected in a more complex network structure (average degree, 54.83) than that of the field populations (average degree, 10.62). Our results provide evidence that environmental factors can influence the bacterial community structure and bacterial relative abundance in ACP populations. This is likely due to the adaptation of ACPs to local environments. The Asian citrus psyllid (ACP) is an important vector of the HLB pathogen, which is a major threat to citrus production around the world. Bacterial communities harbored by insects could be affected by different environmental factors. Understanding these factors that affect the bacterial community of the ACP could be important for the better management of HLB transmission. This work surveyed ACP field populations in mainland China in order to explore the bacterial community diversity of different populations and the potential relationships between environmental factors and predominant symbionts. We have assessed the differences in ACP bacterial communities and identified the prevalent strains in the field. In addition, we compared the bacterial communities of ACP field-collected and laboratory populations. Comparing populations subjected to contrasting conditions could help us to better understand how the ACP adapts to local environmental conditions. Our study provides new insights into how environmental factors influence the bacterial community of the ACP.
昆虫与多种微生物群落相关联，这些微生物群落可对宿主产生重大影响。在此，我们对亚洲柑橘木虱（ACP）——柑橘木虱（半翅目：木虱科）中的细菌群落进行了特征分析，柑橘木虱是毁灭性病原菌“亚洲韧皮杆菌”的主要传播媒介，该病原菌可导致柑橘黄龙病（HLB）。在中国，我们对来自15个田间地点的256只ACP个体以及一个实验室种群进行了测序。结果表明，桂林种群的细菌群落多样性最高（平均香农指数为1.27），郴州种群的丰富度最高（平均Chao1指数为298）。田间采集种群的细菌群落结构存在显著差异，且所有种群都含有被归类为ST - 173菌株的细菌。结构方程模型显示，优势菌株与年均温度呈显著负相关。此外，对感染“亚洲韧皮杆菌”的种群的研究结果表明，总共有140种细菌可能参与与该细菌的相互作用。ACP田间种群的细菌群落比实验室种群更为多样，且一些共生菌的相对出现频率存在显著差异。然而，ACP实验室菌落的细菌群落网络结构（平均度为54.83）比田间种群（平均度为10.62）更为复杂。我们的研究结果证明，环境因素可影响ACP种群中的细菌群落结构和细菌相对丰度。这可能是由于ACP对当地环境的适应。亚洲柑橘木虱（ACP）是HLB病原菌的重要传播媒介，对全球柑橘生产构成重大威胁。昆虫体内的细菌群落可能受到不同环境因素的影响。了解这些影响ACP细菌群落的因素对于更好地管理HLB传播可能具有重要意义。这项工作对中国大陆的ACP田间种群进行了调查，以探索不同种群的细菌群落多样性以及环境因素与主要共生菌之间的潜在关系。我们评估了ACP细菌群落的差异，并确定了田间流行的菌株。此外，我们比较了田间采集的ACP种群和实验室种群的细菌群落。比较处于不同条件下的种群有助于我们更好地了解ACP如何适应当地环境条件。我们的研究为环境因素如何影响ACP的细菌群落提供了新的见解。
