Karpova Evgenia K, Bobrovskikh Margarita A, Deryuzhenko Maksim A, Shishkina Olga D, Gruntenko Nataly E
Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, Russia.
Insects. 2023 Apr 3;14(4):357. doi: 10.3390/insects14040357.
The effect of maternally inherited endosymbiotic bacteria on triglyceride and carbohydrate metabolism, starvation resistance and feeding behavior of females was studied. Eight lines of the same nuclear background were investigated; one had no infection and served as the control, and seven others were infected with different strains pertaining to wMel and wMelCS groups of genotypes. Most of the infected lines had a higher overall lipid content and triglyceride level than the control line and their expression of the gene regulating triglyceride catabolism was reduced. The glucose content was higher in the infected lines compared to that in the control, while their trehalose levels were similar. It was also found that the infection reduced the level of gene expression (coding for enzyme for trehalose synthesis from glucose) and had no effect on gene expression (coding for trehalose degradation enzyme). The infected lines exhibited lower appetite but higher survival under starvation compared to the control. The data obtained may indicate that foster their hosts' energy exchange through increasing its lipid storage and glucose content to ensure the host's competitive advantage over uninfected individuals. The scheme of carbohydrate and lipid metabolism regulation under influence was suggested.
研究了母系遗传的内共生细菌对雌性甘油三酯和碳水化合物代谢、饥饿抗性及摄食行为的影响。对具有相同核背景的8个品系进行了研究；其中一个未感染作为对照，另外7个感染了属于wMel和wMelCS基因型组的不同菌株。大多数感染品系的总体脂质含量和甘油三酯水平高于对照品系，且其调节甘油三酯分解代谢的基因表达降低。与对照相比，感染品系的葡萄糖含量更高，而海藻糖水平相似。还发现感染降低了基因表达水平（编码从葡萄糖合成海藻糖的酶），且对基因表达（编码海藻糖降解酶）没有影响。与对照相比，感染品系的食欲较低，但在饥饿条件下的存活率较高。所获得的数据可能表明，通过增加宿主的脂质储存和葡萄糖含量来促进宿主的能量交换，以确保宿主相对于未感染个体的竞争优势。提出了在影响下碳水化合物和脂质代谢调节的方案。
