DU Hui-Hui, Zhu Fu-Rong, Yang Min, Guo Dong-Qin, Zhao Shun-Xin, Li Qing-Tian, Zhou Nong
Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area's Medicinal Herbs,College of Biological and Food Engineering,Chongqing Three Gorges University Chongqing 404120,China.
College of Pharmacy and Chemistry,Dali University Dali 671000,China.
Zhongguo Zhong Yao Za Zhi. 2021 Feb;46(4):915-922. doi: 10.19540/j.cnki.cjcmm.20201120.102.
The wild resources of Paris polyphylla var. yunnanensis, a secondary endangered medicinal plant, are severely scarce. Introduction and cultivation can alleviate market demand. To screen phosphatolytic bacteria in the rhizosphere soil of P. polyphylla var. yunnanensis and provide data support for the development of high-efficiency microbial fertilizer, in this study, the dilution plate coating method was used to isolate and screen the phosphorus solubilizing bacteria with the ability of mineralizing organic phosphorus from the rhizosphere soil of wild and transplanted varieties of P. polyphylla var. yunnanensis in 10 different locations in Yunnan, Sichuan and Guizhou. After separation and purification, the phosphatolytic capacity was analyzed by qualitative and quantitative analysis. Combined with physiological and biochemical experiments, the strains were identified using 16 S rDNA sequencing analysis. Forty one strains were selected from the rhizosphere soil of P. polyphylla var. yunnanensis from 10 different habitats. Among them, 21 strains were obtained from the rhizosphere soil of the wild variety P. polyphylla var. yunnanensis and 20 strains were obtained from the rhizosphere soil of the transplanted variety. And significance analysis found that 41 organophosphate solubilizing strains had significant differences in their ability to solubilize phosphorus. The amount of phosphate solubilizing was 0.08-67.61 mg·L(-1), the pH value was between 4.27 and 6.82. The phosphatolytic amount of strain Y3-5 was 67.61 mg·L(-1), and the phosphorus increase amount was 57.57 mg·L~(-1). All 41 strains were identified as Gram-positive Bacillus. Combining physiological characteristic and phylogenetic trees, Bacillus mobilis Y3-5 was finally selected as the candidate rhizosphere phosphatolytic bacteria of P. polyphylla var. yunnanensis. The distribution of phosphorus solubilizing bacteria in the rhizosphere soil of P. polyphylla var. yunnanensis was different, and there were significant diffe-rences in phosphorus solubility. Organophosphate-dissolving strain Y3-5 is expected to be a candidate strain of P. polyphylla var. yunnanensis microbial fertilizer.
滇重楼是一种二级濒危药用植物，其野生资源严重稀缺。引种栽培可缓解市场需求。为筛选滇重楼根际土壤中的解磷细菌，为高效微生物肥料的研发提供数据支持，本研究采用稀释平板涂布法，从云南、四川和贵州10个不同地点的野生滇重楼和移栽滇重楼根际土壤中分离筛选具有矿化有机磷能力的解磷细菌。经分离纯化后，通过定性和定量分析对解磷能力进行分析。结合生理生化实验，利用16S rDNA测序分析对菌株进行鉴定。从10个不同生境的滇重楼根际土壤中筛选出41株菌株。其中，从野生滇重楼根际土壤中获得21株，从移栽滇重楼根际土壤中获得20株。显著性分析发现，41株有机磷解磷菌株的解磷能力存在显著差异。解磷量为0.08 - 67.61 mg·L⁻¹，pH值在4.27至6.82之间。菌株Y3 - 5的解磷量为67.61 mg·L⁻¹，增磷量为57.57 mg·L⁻¹。41株菌株均被鉴定为革兰氏阳性芽孢杆菌。结合生理特性和系统发育树，最终筛选出运动芽孢杆菌Y3 - 5作为滇重楼根际解磷细菌候选菌株。滇重楼根际土壤中解磷细菌的分布存在差异，解磷能力也存在显著差异。有机磷溶解菌株Y3 - 5有望成为滇重楼微生物肥料的候选菌株。
