定量 PCR 检测迟钝爱德华菌和芬氏鲺爱德华菌。

Quantitative PCR for Tenacibaculum dicentrarchi and T. finnmarkense.

机构信息

Center of Innovation for Fish Health, Vancouver Island University, Nanaimo, British Columbia, Canada.

Pathobiology, University of Guelph, Guelph, Ontario, Canada.

出版信息

J Fish Dis. 2021 May;44(5):655-659. doi: 10.1111/jfd.13357. Epub 2021 Mar 1.

DOI:10.1111/jfd.13357

PMID:33645841

Abstract

Numerous Tenacibaculum species, including T. dicentrarchi, T. maritimum and T. finnmarkense, are associated with tenacibaculosis in finfish; however, quantitative identification techniques are limited. Quantitative PCR assays were developed to detect T. dicentrarchi and T. finnmarkense. TaqMan assays using 16S rDNA demonstrated low detection limits (0.07-269 bacteria), suitable amplification efficiencies (>86%) and moderate specificity. However, the amplification of isolates with 100% sequence similarity to T. finnmarkense AY7486TD using both the T. finnmarkense and T. dicentrarchi assays indicates that other genes should be investigated. Both assays may help describe the pathogenesis of tenacibaculosis and may aid management practices for the aquaculture industry.

摘要

许多沙雷氏菌属物种，包括 T. dicentrarchi、T. maritimum 和 T. finnmarkense，与鱼类的 Tenacibaculum 病有关；然而，定量鉴定技术有限。开发了定量 PCR 检测方法来检测 T. dicentrarchi 和 T. finnmarkense。使用 16S rDNA 的 TaqMan 检测法具有较低的检测限（0.07-269 个细菌）、合适的扩增效率（>86%）和中等的特异性。然而，使用 T. finnmarkense 和 T. dicentrarchi 检测法对与 T. finnmarkense AY7486TD 具有 100%序列相似性的分离株进行扩增表明，应该研究其他基因。这两种检测方法都可能有助于描述 Tenacibaculum 病的发病机制，并可能有助于水产养殖业的管理实践。