Environmental Engineering Laboratory, Departament d'Enginyeria Química, Universitat Rovira i Virgili, Av. Països Catalans 26, 43007, Tarragona, Catalonia, Spain; Universitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, Unitat de Nutrició Humana. Hospital Sant Joan de Reus, Reus, Spain. Institut d'Investigació Pere Virgili (IISPV). Reus, Spain.
Laboratory of Toxicology and Environmental Health, School of Medicine, IISPV, Universitat Rovira i Virgili, Sant Llorenç 21, 43201, Reus, Catalonia, Spain.
Environ Res. 2021 May;196:110941. doi: 10.1016/j.envres.2021.110941. Epub 2021 Feb 26.
This study was aimed at reconstructing the exposure to bisphenol (BPA) of 60 pregnant women from the EXHES-Spain cohort. A biomonitoring study was conducted by determining BPA levels in urine samples over the three trimesters of pregnancy. Moreover, the correlations between BPA levels and the role of different potential exposure sources, with special emphasis on the dietary intake, were also studied. Urine samples were subjected to dispersive liquid-liquid microextraction and the subsequent analysis via gas chromatography-mass spectrometry. BPA was detected in 76% of the urine samples. A significant decrease of urinary BPA levels was observed along pregnancy, as mean concentrations of creatinine-adjusted BPA were 4.64, 4.84 and 2.51 μg/g in the first, second and third trimester, respectively. This decrease was essentially associated with changes in the dietary habits of the pregnant women, including a lower intake of canned food and drinks. However, the potential role of other pregnancy-related biochemical or physiological factors should not be disregarded. Very interestingly, significant differences in urine BPA levels were found according to the fruit consumption pattern, as women who ate more citrus fruits showed lower BPA concentrations in urine. The reconstructed exposure to BPA was estimated in 0.072, 0.069 and 0.038 μg BPA/kg of body weight/day in the first, second and third trimesters, respectively. These values are far below the temporary tolerable daily intake (t-TDI) established by the EFSA.
本研究旨在通过测定西班牙 EXHES 队列中 60 名孕妇在妊娠三个阶段的尿液样本中的双酚 A（BPA）水平，来重建其 BPA 暴露情况。此外，还研究了 BPA 水平与不同潜在暴露源之间的相关性，特别强调了饮食摄入的作用。尿液样本经分散液液微萃取处理后，通过气相色谱-质谱法进行后续分析。在 76%的尿液样本中检测到了 BPA。随着妊娠的进行，尿液中 BPA 水平呈显著下降趋势，经肌酐校正后的 BPA 平均浓度分别为妊娠第一、第二和第三阶段的 4.64、4.84 和 2.51μg/g。这种下降主要与孕妇饮食习惯的变化有关，包括罐头食品和饮料摄入量的减少。然而，不应忽视其他与妊娠相关的生化或生理因素的潜在作用。有趣的是，根据水果消费模式，尿液 BPA 水平存在显著差异，食用更多柑橘类水果的女性尿液中的 BPA 浓度较低。在妊娠第一、第二和第三阶段，估计的 BPA 暴露量分别为 0.072、0.069 和 0.038μg BPA/kg 体重/天。这些值远低于 EFSA 确定的临时耐受每日摄入量（t-TDI）。
