Biomonitoring of co-exposure to bisphenols by consumers of canned foodstuffs.

For non-occupationally exposed adults, dietary intake is the main route of exposure to bisphenols (BPs), with canned foodstuffs playing a key role. This study was aimed at biomonitoring bisphenol A (BPA) and 5 more BP analogues (BPB, BPE, BPF, BPAF and BPZ) in spot urine and blood samples of a cohort of adults, who followed a diet based on a high consumption of canned food. To the best of our knowledge, this is the first study aimed at assessing the co-exposure of BP analogues in food and biological samples after a two-day duplicate diet study. The estimated total dietary exposure was 0.37 and 0.045 µg/kg body weight/day, for the canned-diet and control groups, respectively. BPA was the compound with the highest concentration in urine in comparison with the values of the remaining BP analogues. A high detection rate of BPA was noted in urine for both groups, 96% for the canned-diet group and 90% for the control group, while in blood it could be only quantified in 6% of the samples. The identification of other analogues was hardly related to diet, so it could be the result of other potential exposure sources, such as personal care products (PCPs) or air inhalation. After 2 days, the excretion of BPA was considerably higher in the canned-diet group subjects than those in the control group (7.02 vs. 1.89 µg/day), confirming that diet and canned foodstuffs are the main route of exposure to BPA. Anyhow, the temporary tolerable daily intake (t-TDI) established by the EFSA was not exceeded, even by those consumers with a diet rich in canned food. Moreover, spot urine samples provided accurate information about exposure and excretion of BPA, being the 4 h, instead of 24 h, the optimal sampling interval, when the collection of spot urine samples is not possible.